RRC ID 49548
Author Sakaue-Sawano A, Yo M, Komatsu N, Hiratsuka T, Kogure T, Hoshida T, Goshima N, Matsuda M, Miyoshi H, Miyawaki A.
Title Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle.
Journal Mol. Cell
Abstract -mediated ubiquitylation. In cultured cells, Fucci(CA) produced a sharp triple color-distinct separation of G1, S, and G2, while Fucci(SCA) permitted a two-color readout of G1 and S/G2. Fucci(CA) applications included tracking the transient G1 phase of rapidly dividing mouse embryonic stem cells and identifying a window for UV-irradiation damage in S phase. These results show that Fucci(CA) is an essential tool for quantitative studies of interphase cell-cycle regulation.
Volume 68(3)
Pages 626-640.e5
Published 2017-11-2
DOI 10.1016/j.molcel.2017.10.001
PII S1097-2765(17)30750-5
PMID 29107535
IF 14.248
DNA material mCherry-hCdt1(1/100)Cy(-)/pcDNA3 (RDB15459) mCherry-hCdt1(1/100)Cy(-)/pCSII-EF (RDB15441) mCherry-hCdt1(1/100)/pCSII-EF (RDB15442) tFucci(SA)2/pCSII-EF (RDB15443) tFucci(SA)2.2/pCSII-EF (RDB15445) tFucci(CA)2/pCSII-EF (RDB15446) tFucci(CA)2.2/pCSII-EF (RDB15448) tFucci(SA)2/pCSII-CMV (RDB15449) tFucci(SA)2.2/pCSII-CMV (RDB15451) tFucci(CA)2/pCSII-CMV (RDB15452) tFucci(CA)2.2/pCSII-CMV (RDB15454).