論文 - 詳細
| RRC ID | 49554 |
|---|---|
| 著者 | Yanagihashi Y, Segawa K, Maeda R, Nabeshima YI, Nagata S. |
| タイトル | Mouse macrophages show different requirements for phosphatidylserine receptor Tim4 in efferocytosis. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
Protein S (ProS) and growth arrest-specific 6 (Gas6) bind to phosphatidylserine (PtdSer) and induce efferocytosis upon binding TAM-family receptors (Tyro3, Axl, and Mer). Here, we produced mouse ProS, Gas6, and TAM-receptor extracellular region fused to IgG fragment crystallizable region in HEK293T cells. ProS and Gas6 bound Ca2+ dependently to PtdSer (Kd 20-40 nM), Mer, and Tyro3 (Kd 15-50 nM). Gas6 bound Axl strongly (Kd < 1.0 nM), but ProS did not bind Axl. Using NIH 3T3-based cell lines expressing a single TAM receptor, we showed that TAM-mediated efferocytosis was determined by the receptor-binding ability of ProS and Gas6. Tim4 is a membrane protein that strongly binds PtdSer. Tim4 alone did not support efferocytosis, but enhanced TAM-dependent efferocytosis. Resident peritoneal macrophages, Kupffer cells, and CD169+ skin macrophages required Tim4 for TAM-stimulated efferocytosis, whereas efferocytosis by thioglycollate-elicited peritoneal macrophages or primary cultured microglia was TAM dependent, but not Tim4 dependent. These results indicate that TAM and Tim4 collaborate for efficient efferocytosis in certain macrophage populations. |
| 巻・号 | 114(33) |
| ページ | 8800-8805 |
| 公開日 | 2017-8-15 |
| DOI | 10.1073/pnas.1705365114 |
| PII | 1705365114 |
| PMID | 28768810 |
| PMC | PMC5565444 |
| MeSH | Animals Calcium-Binding Proteins Carrier Proteins / chemistry* Carrier Proteins / genetics Carrier Proteins / metabolism* HEK293 Cells Humans Intercellular Signaling Peptides and Proteins / chemistry Intercellular Signaling Peptides and Proteins / genetics Intercellular Signaling Peptides and Proteins / metabolism Macrophages, Peritoneal / metabolism* Membrane Proteins / chemistry* Membrane Proteins / genetics Membrane Proteins / metabolism* Mice Mice, Knockout NIH 3T3 Cells |
| IF | 9.412 |
| 引用数 | 18 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pCAG-HIVgp (RDB04394) CSII-EF-MCS (RDB04378) pRSV-Rev (RDB08121). |