| RRC ID |
49561
|
| Author |
Nihongaki Y, Furuhata Y, Otabe T, Hasegawa S, Yoshimoto K, Sato M.
|
| Title |
CRISPR-Cas9-based photoactivatable transcription systems to induce neuronal differentiation.
|
| Journal |
Nat Methods
|
| Abstract |
Our improved CRISPR-Cas9-based photoactivatable transcription systems, CPTS2.0 and Split-CPTS2.0, enable high blue-light-inducible activation of endogenous target genes in various human cell lines. We achieved reversible activation of target genes with CPTS2.0 and induced neuronal differentiation in induced pluripotent stem cells (iPSCs) by upregulating NEUROD1 with Split-CPTS2.0.
|
| Volume |
14(10)
|
| Pages |
963-966
|
| Published |
2017-10-1
|
| DOI |
10.1038/nmeth.4430
|
| PII |
nmeth.4430
|
| PMID |
28892089
|
| MeSH |
CRISPR-Cas Systems*
Cell Differentiation
Cells, Cultured
DNA, Complementary
Fibroblasts
Gene Expression Regulation / physiology
Humans
Light
Luminescent Measurements
Neurons / physiology*
Optogenetics / methods*
Transcription, Genetic
|
| IF |
30.822
|
| Times Cited |
48
|
| Resource |
| DNA material |
pCAGGS (RDB08938). |
| Human and Animal Cells |
HFSKF-II(RCB0698)
201B7(HPS0063)
454E2(HPS0077) |
