RRC ID 49566
Author Kidou S, Ejiri S.
Title Isolation, characterization and mRNA expression of four cDNAs encoding translation elongation factor 1A from rice (Oryza sativa L.).
Journal Plant Mol Biol
Abstract Four different cDNA clones encoding protein synthesis elongation factor 1A, eEF1A, were isolated from rice (Oryza sativa L.). The genes encoded by these cDNAs were designated rice elongation factor 1A genes refa1, refa2, refa3 and refa4. The genes encoded identical eEF-1A polypeptides and shared high amino acid identity with eEF1A of other eukaryotes. Southern blot analysis suggested that some of these refa genes may be organized in a cluster on the same chromosome within a short distance. PCR analysis of rice genomic DNA showed that refa1 and refa4, and refa3 and refa2 are in neighboring locations on the rice genome. The mRNAs of the four refa genes accumulated to nearly equal levels in a variety of tissues and at different stages of growth. Suspension-cultured cells were the most abundant in refa mRNAs. Dormant seeds contained a small amount of the four refa mRNAs. Transcript accumulation was highly induced after seed germination, and the same expression levels were maintained even in old leaf blades of mature plants.
Volume 36(1)
Pages 137-48
Published 1998-1-1
DOI 10.1023/a:1005960721762
PMID 9484469
MeSH Amino Acid Sequence Animals Base Sequence DNA Primers DNA, Complementary / metabolism* Eukaryotic Initiation Factor-1* Evolution, Molecular Gene Library Genes, Plant Humans Molecular Sequence Data Oryza / genetics Oryza / metabolism* Peptide Initiation Factors / biosynthesis* Peptide Initiation Factors / chemistry Peptide Initiation Factors / genetics Phylogeny Polymerase Chain Reaction / methods RNA, Messenger / biosynthesis Sensitivity and Specificity Sequence Alignment Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Transcription, Genetic*
IF 3.302
Times Cited 34
DNA material refa1 (RDB02084)