RRC ID 4964
著者 Inwood WB, Hall JA, Kim KS, Demirkhanyan L, Wemmer D, Zgurskaya H, Kustu S.
タイトル Epistatic effects of the protease/chaperone HflB on some damaged forms of the Escherichia coli ammonium channel AmtB.
ジャーナル Genetics
Abstract The Escherichia coli ammonium channel AmtB is a trimer in which each monomer carries a pore for substrate conduction and a cytoplasmic C-terminal extension of approximately 25 residues. Deletion of the entire extension leaves the protein with intermediate activity, but some smaller lesions in this region completely inactivate AmtB, as do some lesions in its cytoplasmic loops. We here provide genetic evidence that inactivation depends on the essential protease HflB, which appears to cause inactivation not as a protease but as a chaperone. Selection for restored function of AmtB is a positive selection for loss of the ATPase/chaperone activity of HflB and reveals that the conditional lethal phenotype for hflB is cold sensitivity. Deletion of only a few residues from the C terminus of damaged AmtB proteins seems to prevent HflB from acting on them. Either yields the intermediate activity of a complete C-terminal deletion. HflB apparently "tacks" damaged AmtB tails to the adjacent monomers. Knowing that HflB has intervened is prerequisite to determining the functional basis for AmtB inactivation.
巻・号 183(4)
ページ 1327-40
公開日 2009-12-1
DOI 10.1534/genetics.109.103747
PII genetics.109.103747
PMID 19596908
PMC PMC2787424
MeSH ATP-Dependent Proteases / chemistry ATP-Dependent Proteases / metabolism* Amino Acid Sequence Cation Transport Proteins / chemistry Cation Transport Proteins / genetics* Cation Transport Proteins / metabolism Epistasis, Genetic* Escherichia coli / genetics* Escherichia coli / metabolism* Escherichia coli Proteins / chemistry Escherichia coli Proteins / genetics* Escherichia coli Proteins / metabolism* Models, Molecular Molecular Chaperones / chemistry Molecular Chaperones / metabolism* Molecular Sequence Data Mutant Proteins / chemistry Mutant Proteins / genetics* Mutant Proteins / metabolism Protein Conformation Suppression, Genetic
IF 4.015
引用数 10
WOS 分野 GENETICS & HEREDITY
リソース情報
原核生物(大腸菌) JW0441 JW3140 JW4133 JW4132 JW0442