RRC ID 49645
Author Watanabe K, Oda-Yamamizo C, Sage-Ono K, Ohmiya A, Ono M.
Title Alteration of flower colour in Ipomoea nil through CRISPR/Cas9-mediated mutagenesis of carotenoid cleavage dioxygenase 4.
Journal Transgenic Res
Abstract Japanese morning glory, Ipomoea nil, exhibits a variety of flower colours, except yellow, reflecting the accumulation of only trace amounts of carotenoids in the petals. In a previous study, we attributed this effect to the low expression levels of carotenogenic genes in the petals, but there may be other contributing factors. In the present study, we investigated the possible involvement of carotenoid cleavage dioxygenase (CCD), which cleaves specific double bonds of the polyene chains of carotenoids, in the regulation of carotenoid accumulation in the petals of I. nil. Using bioinformatics analysis, seven InCCD genes were identified in the I. nil genome. Sequencing and expression analyses indicated potential involvement of InCCD4 in carotenoid degradation in the petals. Successful knockout of InCCD4 using the CRISPR/Cas9 system in the white-flowered cultivar I. nil cv. AK77 caused the white petals to turn pale yellow. The total amount of carotenoids in the petals of ccd4 plants was increased 20-fold relative to non-transgenic plants. This result indicates that in the petals of I. nil, not only low carotenogenic gene expression but also carotenoid degradation leads to extremely low levels of carotenoids.
Volume 27(1)
Pages 25-38
Published 2018-2-1
DOI 10.1007/s11248-017-0051-0
PII 10.1007/s11248-017-0051-0
PMID 29247330
MeSH CRISPR-Cas Systems Carotenoids / genetics Carotenoids / metabolism Dioxygenases / genetics* Flowers / genetics Flowers / physiology* Gene Expression Regulation, Plant Gene Knockout Techniques Genome, Plant Ipomoea nil / genetics* Ipomoea nil / physiology Mutagenesis Phylogeny Pigmentation / genetics* Pigmentation / physiology Plant Proteins / genetics* Plants, Genetically Modified
IF 1.856
Times Cited 13
Morning Glory AK77