RRC ID 4966
Author Jovanovic G, Engl C, Buck M.
Title Physical, functional and conditional interactions between ArcAB and phage shock proteins upon secretin-induced stress in Escherichia coli.
Journal Mol. Microbiol.
Abstract The phage shock protein (Psp) system found in enterobacteria is induced in response to impaired inner membrane integrity (where the Psp response is thought to help maintain the proton motive force of the cell) and is implicated in the virulence of pathogens such as Yersinia and Salmonella. We provided evidence that the two-component ArcAB system was involved in induction of the Psp response in Escherichia coli and now report that role of ArcAB is conditional. ArcAB, predominantly through the action of ArcA regulated genes, but also via a direct ArcB-Psp interaction, is required to propagate the protein IV (pIV)-dependent psp-inducing signal(s) during microaerobiosis, but not during aerobiosis or anaerobiosis. We show that ArcB directly interacts with the PspB, possibly by means of the PspB leucine zipper motif, thereby allowing cross-communication between the two systems. In addition we demonstrate that the pIV-dependent induction of psp expression in anaerobiosis is independent of PspBC, establishing that PspA and PspF can function as a minimal Psp system responsive to inner membrane stress.
Volume 74(1)
Pages 16-28
Published 2009-10
DOI 10.1111/j.1365-2958.2009.06809.x
PMID 19682256
PMC PMC2764110
MeSH Bacterial Outer Membrane Proteins / genetics Bacterial Outer Membrane Proteins / metabolism* Bacterial Proteins / genetics Bacterial Proteins / metabolism* DNA, Bacterial / genetics Escherichia coli / genetics Escherichia coli / metabolism* Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Gene Expression Regulation, Bacterial Heat-Shock Proteins / genetics Heat-Shock Proteins / metabolism* Membrane Proteins / genetics Membrane Proteins / metabolism* Oxygen Consumption Protein Kinases / genetics Protein Kinases / metabolism* Repressor Proteins / genetics Repressor Proteins / metabolism* Secretin / metabolism Signal Transduction
IF 3.816
Times Cited 21
WOS Category MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Prokaryotes E. coli ME7986(MG1655) JW4364 JW5536 JW1328 JW1213 JW1212 JW2453 JW2181 JW4023 JW4024 JW3933 JW0669