RRC ID |
49667
|
Author |
Tanabe H, Seki M, Itoh M, Deepak A, Lal P, Horiuchi T, Suzuki Y, Kawakami K.
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Title |
Fluorescence-Activated Cell Sorting and Gene Expression Profiling of GFP-Positive Cells from Transgenic Zebrafish Lines.
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Journal |
Methods Mol Biol
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Abstract |
Gene expression profiling is a useful approach for deeper understanding of the specificity of cells, tissues, and organs in the transcriptional level. Recent development of high-throughput next-generation sequence (NGS) allows the RNA-seq method for this profiling. This method provides precise information of transcripts about the quantitation and the structure such as the splicing variants. In this chapter, we describe a method for gene expression profiling of GFP-positive cells from transgenic zebrafish by RNA-seq. We labeled specific cells in the brain with GFP by crossing a Gal4 driver line with the UAS:GFP line, isolated those cells by fluorescence-activated cell sorting (FACS), and analyzed by RNA-seq.
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Volume |
1451
|
Pages |
93-106
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Published |
2016-1-1
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DOI |
10.1007/978-1-4939-3771-4_7
|
PMID |
27464803
|
MeSH |
Animals
Animals, Genetically Modified / genetics
Animals, Genetically Modified / metabolism*
Enhancer Elements, Genetic / genetics*
Flow Cytometry / methods*
Gene Expression Profiling / methods*
Gene Expression Regulation, Developmental / genetics
Gene Expression Regulation, Developmental / physiology
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism*
High-Throughput Nucleotide Sequencing
Zebrafish / genetics
Zebrafish / metabolism*
|
Times Cited |
1
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Resource |
Zebrafish |
|