RRC ID 4972
著者 Liu IF, Annamalai T, Sutherland JH, Tse-Dinh YC.
タイトル Hydroxyl radicals are involved in cell killing by the bacterial topoisomerase I cleavage complex.
ジャーナル J Bacteriol
Abstract Escherichia coli expressing SOS-inducing mutant topoisomerase I was utilized to demonstrate that covalent protein-DNA complex accumulation results in oxidative damage. Hydroxyl radicals were detected following mutant topoisomerase induction. The presence of the Fe(2+) chelator 2,2'-dipyridyl and an iscS mutation affecting Fe-S cluster formation protect against topoisomerase I cleavage complex-mediated cell killing.
巻・号 191(16)
ページ 5315-9
公開日 2009-8-1
DOI 10.1128/JB.00559-09
PII JB.00559-09
PMID 19525344
PMC PMC2725605
MeSH 2,2'-Dipyridyl / pharmacology Anti-Bacterial Agents / pharmacology Arabinose / pharmacology DNA / metabolism DNA Topoisomerases, Type I / genetics DNA Topoisomerases, Type I / metabolism DNA Topoisomerases, Type I / physiology* Escherichia coli / drug effects Escherichia coli / genetics Escherichia coli / metabolism* Hydroxyl Radical / metabolism* Iron Chelating Agents / pharmacology Mutation Norfloxacin / pharmacology
IF 3.006
引用数 18
WOS 分野 MICROBIOLOGY
リソース情報
原核生物(大腸菌) Keio collection JW2514