RRC ID |
5001
|
著者 |
Srionnual S, Yanagida F, Lin LH, Hsiao KN, Chen YS.
|
タイトル |
Weissellicin 110, a newly discovered bacteriocin from Weissella cibaria 110, isolated from plaa-som, a fermented fish product from Thailand.
|
ジャーナル |
Appl Environ Microbiol
|
Abstract |
Weissella cibaria 110, isolated from the Thai fermented fish product plaa-som, was found to produce a bacteriocin active against some gram-positive bacteria. Bacteriocin activity was not eliminated by exposure to high temperatures or catalase but was destroyed by exposure to the proteolytic enzymes proteinase K and trypsin. The bacteriocin from W. cibaria 110 was purified, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that the purified bacteriocin contained one protein band that was approximately 2.5 kDa in size. Mass spectrometry analysis showed the mass of the peptide to be approximately 3,487.8 Da. N-terminal amino acid sequence analysis was performed, and 27 amino acids were identified. Because it has no similarity to other known bacteriocins, this bacteriocin was defined as a new bacteriocin and termed weissellicin 110.
|
巻・号 |
73(7)
|
ページ |
2247-50
|
公開日 |
2007-4-1
|
DOI |
10.1128/AEM.02484-06
|
PII |
AEM.02484-06
|
PMID |
17293526
|
PMC |
PMC1855655
|
MeSH |
Amino Acid Sequence
Bacteriocins / chemistry
Bacteriocins / isolation & purification*
Bacteriocins / pharmacology
Fermentation
Fish Products / microbiology*
Lactobacillus / metabolism*
Molecular Sequence Data
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Temperature
Thailand
|
IF |
4.016
|
引用数 |
76
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
MICROBIOLOGY
|
リソース情報 |
一般微生物 |
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