RRC ID 50011
Author Habib AGK, Sugiura K, Ueno M.
Title Chromosome passenger complex is required for the survival of cells with ring chromosomes in fission yeast.
Journal PLoS ONE
Abstract Ring chromosomes are circular chromosomal abnormalities that have been reported in association with some genetic disorders and cancers. In Schizosaccharomyces pombe, lack of function of protection of telomere 1 (Pot1) or telomerase catalytic subunit (Trt1) results in survivors with circular chromosomes. Hitherto, it is poorly understood how cells with circular chromosomes survive and how circular chromosomes are maintained. Fission yeast Cut17/Bir1, Ark1, Pic1, and Nbl1 is a conserved chromosome passenger complex (CPC) functioning mainly throughout mitosis. Here, using a temperature-sensitive mutant of CPC subunits, we determined that CPC is synthetically lethal in combination with either Pot1 or Trt1. The pot1Δ pic1-T269 double mutant, which has circular chromosomes, showed a high percentage of chromosome mis-segregation and DNA damage foci at 33°C. We furthermore found that neither Shugoshin Sgo2 nor heterochromatin protein Swi6, which contribute to the centromeric localization of CPC, were required for the survival in the absence of Pot1. Both the pot1Δ sgo2Δ and pot1Δ swi6Δ double mutants displayed a high percentage of DNA damage foci, but a low percentage of chromosome mis-segregation, suggesting the link between the high percentage of chromosome mis-segregation and the lethality of the CPC pot1Δ double mutant. Our results suggest that CPC is required for the survival of cells with circular chromosomes and sheds light on the possible roles of CPC in the maintenance of circular chromosomes.
Volume 13(1)
Pages e0190523
Published 2018
DOI 10.1371/journal.pone.0190523
PII PONE-D-17-25263
PMID 29298360
PMC PMC5752009
MeSH Cell Survival Chromosomes, Fungal* DNA Damage DNA, Fungal / genetics Ring Chromosomes* Schizosaccharomyces / genetics*
IF 2.766
Resource
Yeast FY9361 FY24496 FY24764 FY24593 FY24484 FY13784 FY13725