| RRC ID |
50025
|
| Author |
Komiya M, Ito A, Endo M, Hiruma D, Hattori M, Saitoh H, Yoshida M, Ozawa T.
|
| Title |
A genetic screen to discover SUMOylated proteins in living mammalian cells.
|
| Journal |
Sci Rep
|
| Abstract |
Post-translational modification by the Small Ubiquitin-related Modifier (SUMO) is indispensable for diverse biological mechanisms. Although various attempts have been made to discover novel SUMO substrate proteins to unveil the roles of SUMOylation, the reversibility of SUMOylation, and the differences in the SUMOylation level still makes it difficult to explore infrequently-SUMOylated proteins in mammalian cells. Here, we developed a method to screen for mammalian SUMOylated proteins using the reconstitution of split fluorescent protein fragments in living mammalian cells. Briefly, the cells harboring cDNAs of SUMOylated proteins were identified by the reconstituted fluorescence emission and separated by cell sorting. The method successfully identified 36 unreported SUMO2-substrate candidates with distinct intracellular localizations and functions. Of the candidates, we found Atac2, a histone acetyltransferase, was SUMOylated at a lysine 408, and further modified by multiple SUMOs without isoform specificity. Because the present method is applicable to other SUMO isoforms and mammalian cell-types, it could contribute to a deeper understanding of the role of SUMOylation in various biological contexts.
|
| Volume |
7(1)
|
| Pages |
17443
|
| Published |
2017-12-12
|
| DOI |
10.1038/s41598-017-17450-7
|
| PII |
10.1038/s41598-017-17450-7
|
| PMID |
29234079
|
| PMC |
PMC5727073
|
| MeSH |
3T3 Cells
Animals
Blotting, Western
Brain / metabolism
Genetic Techniques*
HEK293 Cells
HeLa Cells
Humans
Immunoprecipitation
Mice
Microscopy, Confocal / methods
Microscopy, Fluorescence / methods
Proteins / chemistry
Proteins / genetics
Proteins / metabolism*
Sumoylation* / physiology
|
| IF |
3.998
|
| Times Cited |
1
|
| Resource |
| DNA material |
pAP3neo cDNA Library Mouse 10T-half (RDB02903) |
