| RRC ID |
50041
|
| Author |
Shan L, Wu C, Chen D, Hou L, Li X, Wang L, Chu X, Hou Y, Wang Z.
|
| Title |
Regulators of alternative polyadenylation operate at the transition from mitosis to meiosis.
|
| Journal |
J Genet Genomics
|
| Abstract |
In the sexually reproductive organisms, gametes are produced by meiosis following a limited mitotic amplification. However, the intrinsic program switching cells from mitotic to meiotic cycle is unclear. Alternative polyadenylation (APA) is a highly conserved means of gene regulation and is achieved by the RNA 3'-processing machinery to generate diverse 3'UTR profiles. In Drosophila spermatogenesis, we observed distinct profiles of transcriptome-wide 3'UTR between mitotic and meiotic cells. In mutant germ cells stuck in mitosis, 3'UTRs of hundreds of genes were consistently shifted. Remarkably, altering the levels of multiple 3'-processing factors disrupted germline's progression to meiosis, indicative of APA's active role in this transition. An RNA-binding protein (RBP) Tut could directly bind 3'UTRs of 3'-processing factors whose expressions were repressed in the presence of Tut-containing complex. Further, we demonstrated that this RBP complex could execute the repression post-transcriptionally by recruiting CCR4/Twin of deadenylation complex. Thus, we propose that an RBP complex regulates the dynamic APA profile to promote the mitosis-to-meiosis transition.
|
| Volume |
44(2)
|
| Pages |
95-106
|
| Published |
2017-2-20
|
| DOI |
10.1016/j.jgg.2016.12.007
|
| PII |
S1673-8527(17)30017-6
|
| PMID |
28190776
|
| MeSH |
3' Untranslated Regions / genetics
Animals
Drosophila melanogaster / cytology
Drosophila melanogaster / genetics
Drosophila melanogaster / metabolism
Gene Expression Profiling
Male
Meiosis*
Mitosis*
Polyadenylation*
RNA-Binding Proteins / metabolism
|
| IF |
5.065
|
| Times Cited |
3
|
| Resource |
| Drosophila |
1972R-1
1972R-2
1109R-1
1109R-2
5222R-1
3642R-1
3642R-3
7697R-1
5970R-2
5970R-3
10228R-1
10228R-4
2163R-1
2163R-2
14213R-1
14213R-2
11486R-3
11486R-4 |
