RRC ID 50051
Author Fan W, Lam SM, Xin J, Yang X, Liu Z, Liu Y, Wang Y, Shui G, Huang X.
Title Drosophila TRF2 and TAF9 regulate lipid droplet size and phospholipid fatty acid composition.
Journal PLoS Genet
Abstract The general transcription factor TBP (TATA-box binding protein) and its associated factors (TAFs) together form the TFIID complex, which directs transcription initiation. Through RNAi and mutant analysis, we identified a specific TBP family protein, TRF2, and a set of TAFs that regulate lipid droplet (LD) size in the Drosophila larval fat body. Among the three Drosophila TBP genes, trf2, tbp and trf1, only loss of function of trf2 results in increased LD size. Moreover, TRF2 and TAF9 regulate fatty acid composition of several classes of phospholipids. Through RNA profiling, we found that TRF2 and TAF9 affects the transcription of a common set of genes, including peroxisomal fatty acid β-oxidation-related genes that affect phospholipid fatty acid composition. We also found that knockdown of several TRF2 and TAF9 target genes results in large LDs, a phenotype which is similar to that of trf2 mutants. Together, these findings provide new insights into the specific role of the general transcription machinery in lipid homeostasis.
Volume 13(3)
Pages e1006664
Published 2017-3
DOI 10.1371/journal.pgen.1006664
PMID 28273089
PMC PMC5362240
MeSH Alleles Amino Acid Motifs Animals Drosophila / genetics* Drosophila / metabolism Drosophila Proteins / metabolism* Fatty Acids / chemistry* Homeostasis Lipids / chemistry* Mutation Oxygen / chemistry Peroxisomes / chemistry Phenotype Phospholipids / chemistry RNA Interference Sequence Analysis, RNA TATA-Binding Protein Associated Factors / metabolism* Telomeric Repeat Binding Protein 2 / metabolism* Transcription Factor TFIID / chemistry Transcription Factor TFIID / metabolism*
IF 5.224
Times Cited 9
Drosophila 1773R-1 2617R-1 3006R-1 3394R-2 3571R-1 3589R-2 3656R-1 3700R-2 3714R-2 4073R-3 4080R-2 5270R-2 5554R-1 5958R-1 6012R-1 6163R-1 6331R-2 11474R-1 12926R-1 13334R-1 13434R-1 13897R-1 14629R-1 14974R-1 15632R-1 15822R-1 17224R-3 30059R-1 30152R-1 11976R-1 31102R-1 6572R-1 31904R-2 11156R-1 11275R-1 12036R-1