RRC ID 50331
Author Ema M, Sogawa K, Watanabe N, Chujoh Y, Matsushita N, Gotoh O, Funae Y, Fujii-Kuriyama Y.
Title cDNA cloning and structure of mouse putative Ah receptor.
Journal Biochem Biophys Res Commun
Abstract Mouse cDNA clones for a putative Ah receptor have been isolated from a cDNA library of mRNA from Hepa-1 cells by an oligonucleotide probe produced by PCR with a pair of primers which was synthesized according to the reported N-terminal sequence of 26 amino acids. The cDNA clones encode a polypeptide of 805 amino acids with a helix-loop-helix motif and with some similarity to a certain region designated PAS of Drosophila Per and Sim, and human Arnt protein. Cotransfection of an expression vector of the Ah receptor with a reporter plasmid pMC6.3k consisting of CYP1A1 promoter and CAT structural gene into CV-1 cells enhanced the CAT expression in response to added 3-methylcholanthrene.
Volume 184(1)
Pages 246-53
Published 1992-4-15
DOI 10.1016/0006-291x(92)91185-s
PII 0006-291X(92)91185-S
PMID 1314586
MeSH Amino Acid Sequence Animals Base Sequence Cell Line Chloramphenicol O-Acetyltransferase / genetics Chloramphenicol O-Acetyltransferase / metabolism Cloning, Molecular Gene Library Humans Methylcholanthrene / pharmacology Mice Molecular Sequence Data Oligonucleotide Probes Polychlorinated Dibenzodioxins / metabolism Polymerase Chain Reaction Receptors, Aryl Hydrocarbon Receptors, Drug / genetics* Recombinant Fusion Proteins / metabolism Restriction Mapping Sequence Homology, Nucleic Acid Transfection
IF 2.705
Times Cited 370
Resource
DNA material Mouse Ah receptor (RDB01364)