| RRC ID |
50340
|
| 著者 |
Nakamoto M, Shibata Y, Ohno K, Usami T, Kamei Y, Taniguchi Y, Todo T, Sakamoto T, Young G, Swanson P, Naruse K, Nagahama Y.
|
| タイトル |
Ovarian aromatase loss-of-function mutant medaka undergo ovary degeneration and partial female-to-male sex reversal after puberty.
|
| ジャーナル |
Mol Cell Endocrinol
|
| Abstract |
Although estrogens have been generally considered to play a critical role in ovarian differentiation in non-mammalian vertebrates, the specific functions of estrogens during ovarian differentiation remain unclear. We isolated two mutants with premature stops in the ovarian aromatase (cyp19a1) gene from an N-ethyl-N-nitrosourea-based gene-driven mutagenesis library of the medaka, Oryzias latipes. In XX mutants, gonads first differentiated into normal ovaries containing many ovarian follicles that failed to accumulate yolk. Subsequently, ovarian tissues underwent extensive degeneration, followed by the appearance of testicular tissues on the dorsal side of ovaries. In the newly formed testicular tissue, strong expression of gsdf was detected in sox9a2-positive somatic cells surrounding germline stem cells suggesting that gsdf plays an important role in testicular differentiation during estrogen-depleted female-to-male sex reversal. We conclude that endogenous estrogens synthesized after fertilization are not essential for early ovarian differentiation but are critical for the maintenance of adult ovaries.
|
| 巻・号 |
460
|
| ページ |
104-122
|
| 公開日 |
2018-1-15
|
| DOI |
10.1016/j.mce.2017.07.013
|
| PII |
S0303-7207(17)30367-2
|
| PMID |
28711606
|
| MeSH |
Amino Acid Sequence
Animals
Aromatase / chemistry
Aromatase / genetics
Base Sequence
Cell Lineage
Down-Regulation / genetics
Estrogens / biosynthesis
Female
Gene Expression Profiling
Loss of Function Mutation / genetics*
Male
Oryzias / genetics*
Ovarian Follicle / pathology
Ovary / pathology*
Sex Determination Processes* / genetics
Sexual Maturation*
Testis / pathology
Up-Regulation / genetics
Vitellogenins / metabolism
|
| IF |
3.871
|
| 引用数 |
17
|
| リソース情報 |
| メダカ |
cyp19a1(K164X) (MT1032)
cyp19a1(Q182X) (MT1033)
d-rR/TOKYO (MT837)
OK-cab (MT830) |
