論文 - 詳細
| RRC ID | 50343 |
|---|---|
| 著者 | Staals RHJ, Agari Y, Maki-Yonekura S, Zhu Y, Taylor DW, van Duijn E, Barendregt A, Vlot M, Koehorst JJ, Sakamoto K, Masuda A, Dohmae N, Schaap PJ, Doudna JA, Heck AJR, Yonekura K, van der Oost J, Shinkai A. |
| タイトル | Structure and activity of the RNA-targeting Type III-B CRISPR-Cas complex of Thermus thermophilus. |
| ジャーナル | Mol Cell |
| Abstract |
The CRISPR-Cas system is a prokaryotic host defense system against genetic elements. The Type III-B CRISPR-Cas system of the bacterium Thermus thermophilus, the TtCmr complex, is composed of six different protein subunits (Cmr1-6) and one crRNA with a stoichiometry of Cmr112131445361:crRNA1. The TtCmr complex copurifies with crRNA species of 40 and 46 nt, originating from a distinct subset of CRISPR loci and spacers. The TtCmr complex cleaves the target RNA at multiple sites with 6 nt intervals via a 5' ruler mechanism. Electron microscopy revealed that the structure of TtCmr resembles a "sea worm" and is composed of a Cmr2-3 heterodimer "tail," a helical backbone of Cmr4 subunits capped by Cmr5 subunits, and a curled "head" containing Cmr1 and Cmr6. Despite having a backbone of only four Cmr4 subunits and being both longer and narrower, the overall architecture of TtCmr resembles that of Type I Cascade complexes. |
| 巻・号 | 52(1) |
| ページ | 135-145 |
| 公開日 | 2013-10-10 |
| DOI | 10.1016/j.molcel.2013.09.013 |
| PMID | 24119403 |
| PMC | PMC4006948 |
| MeSH | Bacterial Proteins / chemistry Bacterial Proteins / genetics Bacterial Proteins / metabolism* CRISPR-Associated Proteins / chemistry CRISPR-Associated Proteins / genetics CRISPR-Associated Proteins / metabolism* Clustered Regularly Interspaced Short Palindromic Repeats High-Throughput Nucleotide Sequencing Microscopy, Electron Models, Molecular Protein Conformation Protein Subunits RNA, Bacterial / chemistry RNA, Bacterial / genetics RNA, Bacterial / metabolism* Ribonucleases / chemistry Ribonucleases / genetics Ribonucleases / metabolism* Sequence Analysis, RNA Spectrometry, Mass, Electrospray Ionization Structure-Activity Relationship Thermus thermophilus / genetics Thermus thermophilus / metabolism* |
| IF | 15.584 |
| 引用数 | 127 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 34 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pUC-cmr6h (RDB15775) PC014162-42 (RDB15779). |