論文 - 詳細
| RRC ID | 50364 |
|---|---|
| 著者 | Uchida N, Takahashi K, Iwasaki R, Yamada R, Yoshimura M, Endo TA, Kimura S, Zhang H, Nomoto M, Tada Y, Kinoshita T, Itami K, Hagihara S, Torii KU. |
| タイトル | Chemical hijacking of auxin signaling with an engineered auxin-TIR1 pair. |
| ジャーナル | Nat Chem Biol |
| Abstract |
The phytohormone auxin indole-3-acetic acid (IAA) regulates nearly all aspects of plant growth and development. Despite substantial progress in our understanding of auxin biology, delineating specific auxin response remains a major challenge. Auxin regulates transcriptional response via its receptors, TIR1 and AFB F-box proteins. Here we report an engineered, orthogonal auxin-TIR1 receptor pair, developed through a bump-and-hole strategy, that triggers auxin signaling without interfering with endogenous auxin or TIR1/AFBs. A synthetic, convex IAA (cvxIAA) hijacked the downstream auxin signaling in vivo both at the transcriptomic level and in specific developmental contexts, only in the presence of a complementary, concave TIR1 (ccvTIR1) receptor. Harnessing the cvxIAA-ccvTIR1 system, we provide conclusive evidence for the role of the TIR1-mediated pathway in auxin-induced seedling acid growth. The cvxIAA-ccvTIR1 system serves as a powerful tool for solving outstanding questions in auxin biology and for precise manipulation of auxin-mediated processes as a controllable switch. |
| 巻・号 | 14(3) |
| ページ | 299-305 |
| 公開日 | 2018-3-1 |
| DOI | 10.1038/nchembio.2555 |
| PII | nchembio.2555 |
| PMID | 29355850 |
| PMC | PMC5812785 |
| MeSH | Arabidopsis / chemistry Arabidopsis / genetics Arabidopsis Proteins / chemistry* Crosses, Genetic F-Box Proteins / chemistry* Gene Expression Regulation, Plant* Indoleacetic Acids / chemistry* Kinetics Mutation Plant Growth Regulators Plant Roots Protein Binding Protein Engineering Receptors, Cell Surface / chemistry* Seedlings Signal Transduction Transgenes |
| IF | 12.587 |
| 引用数 | 25 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 256 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | pEG202 pRS313 |