RRC ID 50400
著者 Zhang H, Kiuchi T, Wang L, Kawamoto M, Suzuki Y, Sugano S, Banno Y, Katsuma S, Shimada T.
タイトル Bm-muted, orthologous to mouse muted and encoding a subunit of the BLOC-1 complex, is responsible for the otm translucent mutation of the silkworm Bombyx mori.
ジャーナル Gene
Abstract "Tanaka's mottled translucent" (otm) is a mutation of the silkworm Bombyx mori that exhibits translucent skin during larval stages. We performed positional cloning of the gene responsible for otm and mapped it to a 364-kb region on chromosome 5 that contains 22 hypothetical protein-coding genes. We performed RNA-seq analysis of the epidermis and fat body of otm larvae and determined that the gene BGIBMGA002619 may be responsible for the otm mutation. BGIBMGA002619 encodes the biosynthesis of lysosome-related organelles complex 1 (BLOC-1) subunit 5, whose ortholog is responsible for the Muted mutant in mouse. Accordingly, we named this gene Bm-muted. We discovered that the expression of Bm-muted in the epidermis and fat body of otm mutants was dramatically suppressed compared with the wild type. We determined the nucleotide sequences of the full-length cDNA and genomic region corresponding to Bm-muted and found that a 538-bp long DNA sequence similar to B. mori transposon Organdy was inserted into the 3' end of the first intron of Bm-muted in two otm strains. The Bm-muted cDNA of otm mutants lacked exon 2, and accordingly generated a premature stop codon in exon 3. In addition, short interfering RNA (siRNA)-mediated knockdown of this gene caused localized partial translucency of larval skin. These data indicate that the mutation in Bm-muted caused the otm-mutant phenotype. We propose that the insertion of Organdy caused a splicing disorder in Bm-muted in the otm mutant, resulting in a null mutation of Bm-muted. This mutation is likely to cause deficiencies in urate granule formation in epidermal cells that result in translucent larval skin.
巻・号 629
ページ 92-100
公開日 2017-9-20
DOI 10.1016/j.gene.2017.07.071
PII S0378-1119(17)30606-6
PMID 28768207
MeSH Animals Bombyx / genetics* Bombyx / growth & development* Carrier Proteins / metabolism Cloning, Molecular Insect Proteins / genetics* Insect Proteins / metabolism Multiprotein Complexes / metabolism Mutation RNA Interference Uric Acid / metabolism Vesicular Transport Proteins / genetics* Vesicular Transport Proteins / metabolism
IF 2.984
引用数 9
リソース情報
カイコ o26 w05