RRC ID 50497
Author Murata T, Honda T, Egawa G, Yamamoto Y, Ichijo R, Toyoshima F, Dainichi T, Kabashima K.
Title Transient elevation of cytoplasmic calcium ion concentration at a single cell level precedes morphological changes of epidermal keratinocytes during cornification.
Journal Sci Rep
Abstract Epidermal keratinocytes achieve sequential differentiation from basal to granular layers, and undergo a specific programmed cell death, cornification, to form an indispensable barrier of the body. Although elevation of the cytoplasmic calcium ion concentration ([Ca2+]i) is one of the factors predicted to regulate cornification, the dynamics of [Ca2+]i in epidermal keratinocytes is largely unknown. Here using intravital imaging, we captured the dynamics of [Ca2+]i in mouse skin. [Ca2+]i was elevated in basal cells on the second time scale in three spatiotemporally distinct patterns. The transient elevation of [Ca2+]i also occurred at the most apical granular layer at a single cell level, and lasted for approximately 40 min. The transient elevation of [Ca2+]i at the granular layer was followed by cornification, which was completed within 10 min. This study demonstrates the tightly regulated elevation of [Ca2+]i preceding the cornification of epidermal keratinocytes, providing possible clues to the mechanisms of cornification.
Volume 8(1)
Pages 6610
Published 2018-4-26
DOI 10.1038/s41598-018-24899-7
PII 10.1038/s41598-018-24899-7
PMID 29700333
PMC PMC5919969
MeSH Animals Calcium / metabolism* Cell Differentiation* Cell Shape Cells, Cultured Cytoplasm / metabolism Epidermal Cells / cytology* Epidermal Cells / metabolism* Fluorescent Antibody Technique Gene Expression Genes, Reporter Ions / metabolism* Keratinocytes / cytology* Keratinocytes / metabolism* Mice Single-Cell Analysis
IF 3.998
Times Cited 4
Mice RBRC05163