| RRC ID |
50497
|
| 著者 |
Murata T, Honda T, Egawa G, Yamamoto Y, Ichijo R, Toyoshima F, Dainichi T, Kabashima K.
|
| タイトル |
Transient elevation of cytoplasmic calcium ion concentration at a single cell level precedes morphological changes of epidermal keratinocytes during cornification.
|
| ジャーナル |
Sci Rep
|
| Abstract |
Epidermal keratinocytes achieve sequential differentiation from basal to granular layers, and undergo a specific programmed cell death, cornification, to form an indispensable barrier of the body. Although elevation of the cytoplasmic calcium ion concentration ([Ca2+]i) is one of the factors predicted to regulate cornification, the dynamics of [Ca2+]i in epidermal keratinocytes is largely unknown. Here using intravital imaging, we captured the dynamics of [Ca2+]i in mouse skin. [Ca2+]i was elevated in basal cells on the second time scale in three spatiotemporally distinct patterns. The transient elevation of [Ca2+]i also occurred at the most apical granular layer at a single cell level, and lasted for approximately 40 min. The transient elevation of [Ca2+]i at the granular layer was followed by cornification, which was completed within 10 min. This study demonstrates the tightly regulated elevation of [Ca2+]i preceding the cornification of epidermal keratinocytes, providing possible clues to the mechanisms of cornification.
|
| 巻・号 |
8(1)
|
| ページ |
6610
|
| 公開日 |
2018-4-26
|
| DOI |
10.1038/s41598-018-24899-7
|
| PII |
10.1038/s41598-018-24899-7
|
| PMID |
29700333
|
| PMC |
PMC5919969
|
| MeSH |
Animals
Calcium / metabolism*
Cell Differentiation*
Cell Shape
Cells, Cultured
Cytoplasm / metabolism
Epidermal Cells / cytology*
Epidermal Cells / metabolism*
Fluorescent Antibody Technique
Gene Expression
Genes, Reporter
Ions / metabolism*
Keratinocytes / cytology*
Keratinocytes / metabolism*
Mice
Single-Cell Analysis
|
| IF |
3.998
|
| 引用数 |
4
|
| リソース情報 |
| 実験動物マウス |
RBRC05163 |
