RRC ID 50517
Author Ando R, Mizuno H, Miyawaki A.
Title Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting.
Journal Science
Abstract The observation of the regulation of fast protein dynamics in a cellular context requires the development of reliable technologies. Here, a signal regulation cascade reliant on the stimulus-dependent acceleration of the bidirectional flow of mitogen-activated protein kinase (extracellular signal-regulated kinase) across the nuclear envelope was visualized by reversible protein highlighting. Light-induced conversion between the bright and dark states of a monomeric fluorescent protein engineered from a novel coral protein was employed. Because of its photochromic properties, the protein could be highlighted, erased, and highlighted again in a nondestructive manner, allowing direct observation of regulated fast nucleocytoplasmic shuttling of key signaling molecules.
Volume 306(5700)
Pages 1370-3
Published 2004-11-19
DOI 10.1126/science.1102506
PII 306/5700/1370
PMID 15550670
MeSH Active Transport, Cell Nucleus Amino Acid Sequence Animals Anthozoa COS Cells Cell Nucleus / metabolism* Cytoplasm / metabolism* Epidermal Growth Factor / pharmacology Fluorescence HeLa Cells Humans Hydrogen-Ion Concentration Light Luminescent Proteins / chemistry Luminescent Proteins / metabolism* MAP Kinase Signaling System Microscopy, Confocal Mitogen-Activated Protein Kinase 3 / metabolism* Molecular Sequence Data Nuclear Envelope / metabolism* Phosphorylation Protein Transport Recombinant Proteins / chemistry Recombinant Proteins / metabolism Transfection beta Karyopherins / metabolism
IF 41.846
Times Cited 572
DNA material Dronpa/pRSETB (RDB15256) 22G/pRSETB (RDB15255) CoralHue™ humanized Dronpa-Green 1 (phDG1-MNL) (RDB18266) CoralHue™ Dronpa-Green 1 (pDG1-S1) (RDB18267) CoralHue™ Dronpa-Green 1 (pDG1-MC1) (RDB18268) CoralHue™ Dronpa-Green 1 (pDG1-MN1) (RDB18269) CoralHue™ humanized Dronpa-Green 1 (phDG1-MCL) (RDB18270) CoralHue™ Dronpa-Green 3 (pDG3-S1) (RDB18297)