論文 - 詳細
| RRC ID | 50517 |
|---|---|
| 著者 | Ando R, Mizuno H, Miyawaki A. |
| タイトル | Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting. |
| ジャーナル | Science |
| Abstract |
The observation of the regulation of fast protein dynamics in a cellular context requires the development of reliable technologies. Here, a signal regulation cascade reliant on the stimulus-dependent acceleration of the bidirectional flow of mitogen-activated protein kinase (extracellular signal-regulated kinase) across the nuclear envelope was visualized by reversible protein highlighting. Light-induced conversion between the bright and dark states of a monomeric fluorescent protein engineered from a novel coral protein was employed. Because of its photochromic properties, the protein could be highlighted, erased, and highlighted again in a nondestructive manner, allowing direct observation of regulated fast nucleocytoplasmic shuttling of key signaling molecules. |
| 巻・号 | 306(5700) |
| ページ | 1370-3 |
| 公開日 | 2004-11-19 |
| DOI | 10.1126/science.1102506 |
| PII | 306/5700/1370 |
| PMID | 15550670 |
| MeSH | Active Transport, Cell Nucleus Amino Acid Sequence Animals Anthozoa COS Cells Cell Nucleus / metabolism* Cytoplasm / metabolism* Epidermal Growth Factor / pharmacology Fluorescence HeLa Cells Humans Hydrogen-Ion Concentration Light Luminescent Proteins / chemistry Luminescent Proteins / metabolism* MAP Kinase Signaling System Microscopy, Confocal Mitogen-Activated Protein Kinase 3 / metabolism* Molecular Sequence Data Nuclear Envelope / metabolism* Phosphorylation Protein Transport Recombinant Proteins / chemistry Recombinant Proteins / metabolism Transfection beta Karyopherins / metabolism |
| IF | 41.846 |
| 引用数 | 572 |
| リソース情報 | |
| 遺伝子材料 | Dronpa/pRSETB (RDB15256) 22G/pRSETB (RDB15255) CoralHue™ humanized Dronpa-Green 1 (phDG1-MNL) (RDB18266) CoralHue™ Dronpa-Green 1 (pDG1-S1) (RDB18267) CoralHue™ Dronpa-Green 1 (pDG1-MC1) (RDB18268) CoralHue™ Dronpa-Green 1 (pDG1-MN1) (RDB18269) CoralHue™ humanized Dronpa-Green 1 (phDG1-MCL) (RDB18270) CoralHue™ Dronpa-Green 3 (pDG3-S1) (RDB18297) |