論文 - 詳細
| RRC ID | 50645 |
|---|---|
| 著者 | Shiota M, Naya M, Yamamoto T, Hishiki T, Tani T, Takahashi H, Kubo A, Koike D, Itoh M, Ohmura M, Kabe Y, Sugiura Y, Hiraoka N, Morikawa T, Takubo K, Suina K, Nagashima H, Sampetrean O, Nagano O, Saya H, Yamazoe S, Watanabe H, Suematsu M. |
| タイトル | Gold-nanofève surface-enhanced Raman spectroscopy visualizes hypotaurine as a robust anti-oxidant consumed in cancer survival. |
| ジャーナル | Nat Commun |
| Abstract |
Gold deposition with diagonal angle towards boehmite-based nanostructure creates random arrays of horse-bean-shaped nanostructures named gold-nanofève (GNF). GNF generates many electromagnetic hotspots as surface-enhanced Raman spectroscopy (SERS) excitation sources, and enables large-area visualization of molecular vibration fingerprints of metabolites in human cancer xenografts in livers of immunodeficient mice with sufficient sensitivity and uniformity. Differential screening of GNF-SERS signals in tumours and those in parenchyma demarcated tumour boundaries in liver tissues. Furthermore, GNF-SERS combined with quantum chemical calculation identified cysteine-derived glutathione and hypotaurine (HT) as tumour-dominant and parenchyma-dominant metabolites, respectively. CD44 knockdown in cancer diminished glutathione, but not HT in tumours. Mechanisms whereby tumours sustained HT under CD44-knockdown conditions include upregulation of PHGDH, PSAT1 and PSPH that drove glycolysis-dependent activation of serine/glycine-cleavage systems to provide one-methyl group for HT synthesis. HT was rapidly converted into taurine in cancer cells, suggesting that HT is a robust anti-oxidant for their survival under glutathione-suppressed conditions. |
| 巻・号 | 9(1) |
| ページ | 1561 |
| 公開日 | 2018-4-19 |
| DOI | 10.1038/s41467-018-03899-1 |
| PII | 10.1038/s41467-018-03899-1 |
| PMID | 29674746 |
| PMC | PMC5908798 |
| MeSH | Animals Antioxidants / chemistry* Antioxidants / metabolism* Female Glutathione / metabolism Gold / chemistry* Gold / metabolism Humans Liver / chemistry Liver / metabolism Male Metal Nanoparticles / chemistry* Mice Mice, Inbred C57BL Mice, SCID Neoplasms / genetics Neoplasms / metabolism* Spectrum Analysis, Raman / instrumentation Spectrum Analysis, Raman / methods* Taurine / analogs & derivatives* Taurine / chemistry Taurine / metabolism Transaminases / genetics Transaminases / metabolism |
| IF | 12.121 |
| 引用数 | 26 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MCF7(RCB1904) HCT116(RCB2979) |