RRC ID 50725
著者 Wang HF, Li QL, Xiao M, Zhang YG, Zhou XK, Narsing Rao MP, Duan YQ, Li WJ.
タイトル Streptomyces capparidis sp. nov., a novel endophytic actinobacterium isolated from fruits of Capparis spinosa L.
ジャーナル Int J Syst Evol Microbiol
Abstract A novel endophytic actinobacterial strain, designated EGI 6500195T, was isolated from fruits of Capparis spinosa. Growth occurred at 10-45 °C (optimum 30 °C), at pH 6-8 (optimum pH 7) and in the presence of 0-1 % (w/v) NaCl. Strain EGI 6500195T shared highest 16S rRNA gene sequence similarity (97.74 %) with Streptomyces vitaminophilus DSM 41686T and less than 97 % sequence similarity with other members of the genus Streptomyces. The diagnostic amino acid in the peptidoglycan was ll-diaminopimelic acid. Whole-cell hydrolysates contained glucose, ribose, fructose and mannose. The predominant menaquinones were MK-9(H6) and MK-9(H8). The polar lipid profile of strain EGI 6500195T included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylinositol, phosphatidylcholine, three unknown phospholipids, an unknown aminophospholipid and an unknown aminolipid. The cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0, iso-C15 : 0, iso-C16 : 0, anteiso-C17 : 1ω9c, summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B) and iso-C17 : 1ω9c. The DNA G+C content of strain EGI 6500195T was 74.1 mol%. The level of DNA-DNA relatedness between strain EGI 6500195T and Streptomyces. vitaminophilus DSM 41686T was 14.1±3.5 %. On the basis of the phenotypic, phylogenetic, chemotaxonomic and DNA-DNA hybridization data, strain EGI 6500195T represents a novel species of the genus Streptomyces, for which the name Streptomyces capparidis sp. nov. is proposed. The type strain is EGI 6500195T (=DSM 42145T=JCM 30089T).
巻・号 67(1)
ページ 133-137
公開日 2017-1-1
DOI 10.1099/ijsem.0.001586
PMID 27902318
MeSH Bacterial Typing Techniques Base Composition Capparis / microbiology* China DNA, Bacterial / genetics Diaminopimelic Acid / chemistry Fatty Acids / chemistry Fruit / microbiology* Nucleic Acid Hybridization Peptidoglycan / chemistry Phospholipids / chemistry Phylogeny* RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Streptomyces / classification* Streptomyces / genetics Streptomyces / isolation & purification Vitamin K 2 / analogs & derivatives
IF 2.415
引用数 4
リソース情報
一般微生物 JCM 30089 JCM 6307