| RRC ID |
50773
|
| Author |
Teng D, Kawai K, Mikajiri S, Hagura Y.
|
| Title |
Stabilization of freeze-dried Lactobacillus paracasei subsp. paracasei JCM 8130T with the addition of disaccharides, polymers, and their mixtures.
|
| Journal |
Biosci Biotechnol Biochem
|
| Abstract |
Although freeze-drying is a widely used dehydration technique for the stabilizing of unstable lactic acid bacteria, Lactobacillus paracasei subsp. paracasei JCM 8130T (L. paracasei) is destabilized after freeze-drying and subsequent storage. In order to improve the stability of freeze-dried L. paracasei, effects of disaccharides (sucrose and trehalose), polymers (maltodextrin; MD and bovine serum albumin; BSA), and their mixtures on the survival rate of freeze-dried L. paracasei were investigated. The survival rate of non-additive sample decreased slightly after freeze-drying but decreased drastically after subsequent storage at 37 °C for 4 weeks. The reduction was diminished by the addition of disaccharides and polymers. The stabilizing effect of disaccharides was not affected by the co-addition of MD. In contrast, the disaccharide-BSA mixtures had a synergistic stabilizing effect, and the survival rates were largely maintained even after storage. It is suggested that the synergistic effect originates from the conformational stabilization of the dehydrated bacteria.
|
| Volume |
81(4)
|
| Pages |
768-773
|
| Published |
2017-4-1
|
| DOI |
10.1080/09168451.2017.1279852
|
| PMID |
28103748
|
| MeSH |
Animals
Cattle
Disaccharides / chemistry
Food Microbiology*
Freeze Drying
Humans
Lacticaseibacillus paracasei / chemistry
Lacticaseibacillus paracasei / drug effects*
Polysaccharides / pharmacology
Serum Albumin, Bovine / pharmacology
Sucrose / pharmacology
Temperature
Trehalose / pharmacology
Water / chemistry
|
| IF |
1.516
|
| Times Cited |
3
|
| Resource |
| General Microbes |
JCM 8130 |
