RRC ID 50854
Author Aiba Y, Ishikawa H, Tokunaga M, Komatsu Y.
Title Anti-Helicobacter pylori activity of non-living, heat-killed form of lactobacilli including Lactobacillus johnsonii No.1088.
Journal FEMS Microbiol Lett
Abstract Some strains of lactic acid bacteria are reported to inhibit the growth of Helicobacter pylori and proposed to be useful to support so-called triple therapy for H. pylori. Although most strains must be alive to exert their anti-H. pylori activity, some lactobacilli strains are effective even when dead. One possible underlying mechanism of such an activity of non-living lactobacilli is reportedly co-aggregation with H. pylori. In this study, we found that a non-living heat-killed form of Lactobacillus johnsonii No.1088 (HK-LJ88) and also that of some other lactobacilli inhibited the growth of H. pylori in vitro. Furthermore, the number of H. pylori in the infected stomach of germ-free mice was significantly decreased by the repeated oral administration of HK-LJ88. Observation by scanning electron microscopy revealed that no co-aggregation had occurred between H. pylori and HK-LJ88; instead, deformations of H. pylori (e.g. disappearance of spiral, bending of cell body, coccoid formation, degradations, etc.) appeared after incubation for 24 h with HK-LJ88. These results suggest that HK-LJ88 inhibited H. pylori activity probably not by co-aggregation but by some unknown mechanism involving HK-LJ88's cell surface molecules and that even non-living lactobacilli are possibly useful to support H. pylori eradication therapy.
Volume 364(11)
Published 2017-6-15
DOI 10.1093/femsle/fnx102
PII 3827362
PMID 28505287
MeSH Animals Antibiosis* Disease Models, Animal Female Helicobacter Infections / therapy Helicobacter pylori / growth & development* Hot Temperature Lactobacillus / classification Lactobacillus / physiology* Lactobacillus johnsonii / physiology* Male Mice Mice, Inbred BALB C Probiotics Stomach / microbiology
IF 1.987
Times Cited 9
General Microbes JCM 1132 JCM 1059 JCM 1173 JCM 1131 JCM 2012 JCM 1134 JCM 1558 JCM 1149 JCM 1112