論文 - 詳細
| RRC ID | 50889 |
|---|---|
| 著者 | Ohara-Nemoto Y, Nakasato M, Shimoyama Y, Baba TT, Kobayakawa T, Ono T, Yaegashi T, Kimura S, Nemoto TK. |
| タイトル | Degradation of Incretins and Modulation of Blood Glucose Levels by Periodontopathic Bacterial Dipeptidyl Peptidase 4. |
| ジャーナル | Infect Immun |
| Abstract |
Severe periodontitis is known to aggravate diabetes mellitus, though molecular events related to that link have not been fully elucidated. Porphyromonas gingivalis, a major pathogen of periodontitis, expresses dipeptidyl peptidase 4 (DPP4), which is involved in regulation of blood glucose levels by cleaving incretins in humans. We examined the enzymatic characteristics of DPP4 from P. gingivalis as well as two other periodontopathic bacteria, Tannerella forsythia and Prevotella intermedia, and determined whether it is capable of regulating blood glucose levels. Cell-associated DPP4 activity was found in those microorganisms, which was effectively suppressed by inhibitors of human DPP4, and molecules sized 73 kDa in P. gingivalis, and 71 kDa in T. forsythia and P. intermedia were immunologically detected. The kcat/Km values of recombinant DPP4s ranged from 721 ± 55 to 1,283 ± 23 μM-1s-1 toward Gly-Pro-4-methylcoumaryl-7-amide (MCA), while those were much lower for His-Ala-MCA. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis showed His/Tyr-Ala dipeptide release from the N termini of incretins, glucagon-like peptide 1 (GLP-1) and glucose-dependent insulinotropic polypeptide, respectively, with the action of microbial DPP4. Moreover, intravenous injection of DPP4 into mice decreased plasma active GLP-1 and insulin levels, accompanied by a substantial elevation in blood glucose over the control after oral glucose administration. These results are the first to show that periodontopathic bacterial DPP4 is capable of modulating blood glucose levels the same as mammalian DPP4; thus, the incidence of periodontopathic bacteremia may exacerbate diabetes mellitus via molecular events of bacterial DPP4 activities. |
| 巻・号 | 85(9) |
| 公開日 | 2017-9-1 |
| DOI | 10.1128/IAI.00277-17 |
| PII | IAI.00277-17 |
| PMID | 28630069 |
| PMC | PMC5563571 |
| MeSH | Animals Blood Glucose* Dipeptidyl Peptidase 4 / genetics Dipeptidyl Peptidase 4 / metabolism* Female Gastric Inhibitory Polypeptide / metabolism Glucagon-Like Peptide 1 / metabolism Incretins / metabolism* Insulin / blood Mice, Inbred C57BL Porphyromonas gingivalis / enzymology* Prevotella intermedia / enzymology* Proteolysis Recombinant Proteins / genetics Recombinant Proteins / metabolism Tannerella forsythia / enzymology* |
| IF | 3.201 |
| 引用数 | 10 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 9 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 10827 |