論文 - 詳細
| RRC ID | 50932 |
|---|---|
| 著者 | Gupta V, Singh PK, Korpole S, Tanuku NRS, Pinnaka AK. |
| タイトル | Bacillus mangrovi sp. nov., isolated from a sediment sample from a mangrove forest. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
A facultatively anaerobic, endospore forming, alkali-tolerant, Gram-stain-positive, motile, rod-shaped bacterium, designated strain AK61T, was isolated from a sediment sample collected from Coringa mangrove forest, India. Colonies were circular, 1.5 mm in diameter, shiny, smooth, yellowish and convex with entire margins after 48 h growth at 30 °C. Growth occurred at 15-42 °C, with 0-3 % (w/v) NaCl and at pH 6-9. AK61T was positive for amylase activity and negative for oxidase, catalase, aesculinase, caseinase, cellulase, DNase, gelatinase, lipase and urease activities. The fatty acids were dominated by branched types with iso- and anteiso- saturated fatty acids with a high abundance of iso-C14 : 0, iso-C15 : 0, anteiso-C15 : 0 and iso-C16 : 0; the cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid; and MK-7 was the major menaquinone. DNA-DNA hybridization between AK61T and Bacillus indicus MTCC 4374T and between AK61T and Bacillus indicus KCTC 3880 showed relatedness of 37.99 and 33.32 % respectively. The DNA G+C content of AK61T was 44 mol%. The results of a blast sequence similarity search based on 16S rRNA gene sequences indicated that Bacillus cibi and Bacillus indicus were the nearest phylogenetic neighbours, with a pair-wise sequence similarity of 97.69 and 97.55 % respectively. The results of phylogenetic analysis indicated that AK61T was clustered with Bacillus idriensis and Bacillus indicus. On the basis of its phenotypic characteristics and phylogenetic inference, AK61T represents a novel species of the genus Bacillus, for which the name Bacillus mangrovi sp. nov. is proposed. The type strain is AK61T (=JCM 31087T=MTCC 12015T=KCTC 33872T). |
| 巻・号 | 67(7) |
| ページ | 2219-2224 |
| 公開日 | 2017-7-1 |
| DOI | 10.1099/ijsem.0.001928 |
| PMID | 28714845 |
| MeSH | Avicennia / microbiology* Bacillus / classification* Bacillus / genetics Bacillus / isolation & purification Bacterial Typing Techniques Base Composition Cell Wall / chemistry DNA, Bacterial / genetics Diaminopimelic Acid / chemistry Fatty Acids / chemistry India Nucleic Acid Hybridization Peptidoglycan / chemistry Phylogeny* RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Vitamin K 2 / analogs & derivatives Vitamin K 2 / chemistry Wetlands* |
| IF | 2.415 |
| 引用数 | 3 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 一般微生物 | JCM 31087 |