RRC ID |
50950
|
著者 |
Sorokin DY, Kublanov IV, Khijniak TV.
|
タイトル |
Natronospira proteinivora gen. nov., sp. nov, an extremely salt-tolerant, alkaliphilic gammaproteobacterium from hypersaline soda lakes.
|
ジャーナル |
Int J Syst Evol Microbiol
|
Abstract |
Brine samples from Kulunda Steppe soda lakes (Altai, Russia) were inoculated into a hypersaline alkaline mineral medium with β-keratin (chicken feather) as a substrate. The micro-organisms dominating the enrichment culture were isolated by limiting serial dilution on the same medium with casein as a substrate. The cells of strain BSker1T were motile, curved rods. The strain was an obligately aerobic heterotroph utilizing proteins and peptides as growth substrates. The isolate was an obligate alkaliphile with a pH range for growth from pH 8.5 to 10.25 (optimum at pH 9.5), and it was extremely salt tolerant, growing with between 1 and 4.5 M total Na+ (optimally at 2-2.5 M). BSker1T had a unique composition of polar lipid fatty acids, dominated by two C17 species. The membrane polar lipids included multiple unidentified phospholipids and two aminolipids. According to phylogenetic analysis of the 16S rRNA gene sequence, the isolate forms a novel branch within the family Ectothiorhodospiraceae (class Gammaproteobacteria) with the highest sequence similarity to the members of this family being 91 %. On the basis of distinct phenotypic and genotypic properties, strain BSker1T (=JCM 31341T=UNIQEM U1008T) is proposed to be classified as a representative of a novel genus and species, Natronospira proteinivora gen. nov., sp. nov.
|
巻・号 |
67(8)
|
ページ |
2604-2608
|
公開日 |
2017-8-1
|
DOI |
10.1099/ijsem.0.001983
|
PMID |
28758627
|
MeSH |
Alkalies*
Bacterial Typing Techniques
Base Composition
DNA, Bacterial / genetics
Ectothiorhodospiraceae / classification*
Ectothiorhodospiraceae / genetics
Ectothiorhodospiraceae / isolation & purification
Fatty Acids / chemistry
Lakes / microbiology
Phospholipids / chemistry
Phylogeny*
RNA, Ribosomal, 16S / genetics
Russia
Salinity*
Sequence Analysis, DNA
Water Microbiology*
|
IF |
2.415
|
引用数 |
6
|
リソース情報 |
一般微生物 |
JCM 31341 |