| RRC ID |
50952
|
| 著者 |
Ojha AK, Verma A, Pal Y, Bhatt D, Mayilraj S, Krishnamurthi S.
|
| タイトル |
Marinomonas epiphytica sp. nov., isolated from a marine intertidal macroalga.
|
| ジャーナル |
Int J Syst Evol Microbiol
|
| Abstract |
A novel Gram-stain-negative, aerobic marine bacterial strain, SAB-3T, was isolated from brown macroalgae (Dictyota sp.) growing in the Arabian sea, Goa, India. The strain grew optimally at 30 °C, with 2.0-4.0 % (w/v) NaCl and at pH 7.0 on marine agar medium. Strain SAB-3T was unable to hydrolyse aesculin and did not grow in the presence of rifamycin but showed resistance to antibiotics such as cefadroxil and co-trimoxazole. The major fatty acids were summed feature 8 (C18 : 1ω7c/C18 : 1ω6c), summed feature 3 (C16 : 1ω7c/C16 : 1ω6c) and C16 : 0, and Q-8 was the major ubiquinone. The major polar lipids were phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content was 41.0 mol%. 16S rRNA gene sequencing and phylogenetic analysis indicated that the strain was a member of the genus Marinomonas with Marinomonas aquiplantarum IVIA-Po-159T (97.6 % similarity), Marinomonas posidonica IVIA-Po-181T (97.5 %) and Marinomonas dokdonensis DSM 17202T (97.4 %) as the closest relatives. Whole genome relatedness determined through DNA-DNA hybridization revealed values of 40-50 % (below the 70 % threshold recommended for species delineation) with the above three species, thus confirming it as representing a distinct and novel species of the genus Marinomonas for which the name Marinomonas epiphytica sp. nov. is proposed. The type strain is SAB-3T (=JCM 31365T=KCTC 52293T=MTCC 12569T).
|
| 巻・号 |
67(8)
|
| ページ |
2746-2751
|
| 公開日 |
2017-8-1
|
| DOI |
10.1099/ijsem.0.002014
|
| PMID |
28771118
|
| MeSH |
Bacterial Typing Techniques
Base Composition
DNA, Bacterial / genetics
Fatty Acids / chemistry
India
Marinomonas / classification*
Marinomonas / genetics
Marinomonas / isolation & purification
Nucleic Acid Hybridization
Phosphatidylethanolamines / chemistry
Phosphatidylglycerols / chemistry
Phylogeny*
RNA, Ribosomal, 16S / genetics
Seaweed / microbiology*
Sequence Analysis, DNA
Ubiquinone / chemistry
|
| IF |
2.415
|
| 引用数 |
5
|
| リソース情報 |
| 一般微生物 |
JCM 31365 |
