RRC ID 51003
Author Sujarit K, Sujada N, Kudo T, Ohkuma M, Pathom-Aree W, Lumyong S.
Title Pseudonocardia thailandensis sp. nov., an actinomycete isolated from a subterranean termite nest.
Journal Int. J. Syst. Evol. Microbiol.
Abstract A novel Gram-stain-positive bacterium designated CMU-NKS-70T was isolated from a subterranean termite nest and characterized using a polyphasic approach. The strain exhibited branching, pinkish-cream aerial mycelium and cream-brown substrate mycelium, and formed chains of rod-like spores. The 16S rRNA gene sequence analyses indicated that strain CMU-NKS-70T belonged to the genus Pseudonocardia, showing high similarity with Pseudonocardia oroxyli D10T (98.9 % 16S rRNA gene sequence similarity), Pseudonocardia xishanensis YIM 63638T (98.9 %) and Pseudonocardia kujensis A4038T (98.5 %). However, DNA-DNA relatedness values between strains CMU-NKS-70T and the closest phylogenetically related species ranged from 40.5±2.9 to 48.6±0.7 %. Whole-cell hydrolysates of strain CMU-NKS-70T consisted of meso-diaminopimelic acid, glucose, galactose, arabinose, mannose, ribose and rhamnose. The predominant menaquinone was MK-8(H4). The major cellular fatty acids (>10 %) were iso-C16 : 0, C16 : 0, C16 : 1ω7c and/or iso-C15 : 0 2-OH and 10-methyl C16 : 0. The polar lipids detected were phosphatidylethanolamine, phosphatidylmethylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, three unidentified glycolipids and two unidentified phospholipids. The G+C content of genomic DNA was 71.9 mol%. The physiological and biochemical properties also supported the phenotypic distinction of strain CMU-NKS-70T from its closely related species. On the basis of evidence from this study using a polyphasic approach, strain CMU-NKS-70T represents a novel species of the genus Pseudonocardia for which the name Pseudonocardia thailandensis sp. nov. is proposed. The type strain is CMU-NKS-70T (=JCM 31292T=TBRC 2000T).
Volume 67(8)
Pages 2773-2778
Published 2017-8
DOI 10.1099/ijsem.0.002017
PMID 28853680
MeSH Actinomycetales / classification* Actinomycetales / genetics Actinomycetales / isolation & purification Animals Bacterial Typing Techniques Base Composition DNA, Bacterial / genetics Diaminopimelic Acid / chemistry Fatty Acids / chemistry Glycolipids / chemistry Isoptera / microbiology* Nucleic Acid Hybridization Phospholipids / chemistry Phylogeny* RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Thailand Vitamin K 2 / analogs & derivatives Vitamin K 2 / chemistry
IF 1.932
General Microbes JCM 31292 JCM 13909 JCM 17906