| RRC ID |
51051
|
| 著者 |
Asakawa Y, Takesue N, Asano S, Shimotsu S, Iijima K, Suzuki K, Motoyama Y, Aizawa M.
|
| タイトル |
Lactobacillus curtus sp. nov., isolated from beer in Finland.
|
| ジャーナル |
Int J Syst Evol Microbiol
|
| Abstract |
A Gram-stain-positive, catalase-negative and short-rod-shaped organism, designated VTT E-94560, was isolated from beer in Finland and deposited in the VTT culture collection as a strain of Lactobacillus rossiae. However, the results of 16S rRNA gene sequence analysis showed that VTT E-94560 was only related to Lactobacillus rossiae JCM 16176T with 97.0 % sequence similarity, lower than the 98.7 % regarded as the boundary for the species differentiation. Additional phylogenetic studies on the pheS gene, rpoA gene and 16S-23S rRNA internally transcribed spacer region further reinforced the taxonomically independent status of VTT E-94560 and its related Lactobacillus species including L. rossiae and Lactobacillus siliginis. Strain VTT E-94560 also exhibited several differences in its carbohydrate fermentation profiles from those related Lactobacillus species. In addition, DNA-DNA relatedness between VTT E-94560 and these two type strains was 4 % (L. rossiae JCM 16176T) and 12 % (L. siliginins JCM 16155T), respectively, which were lower than the 70 % cut-off for general species delineation, indicating that these three strains are not taxonomically identical at the species level. These studies revealed that VTT E-94560 represents a novel species, for which the name Lactobacillus curtus sp. nov. is proposed. The type strain is VTT E-94560T (=JCM 31185T).
|
| 巻・号 |
67(10)
|
| ページ |
3899-3906
|
| 公開日 |
2017-10-1
|
| DOI |
10.1099/ijsem.0.002220
|
| PMID |
28895518
|
| MeSH |
Bacterial Typing Techniques
Base Composition
Beer / microbiology*
DNA, Bacterial / genetics
Finland
Food Microbiology*
Genes, Bacterial
Lactobacillus / classification*
Lactobacillus / genetics
Lactobacillus / isolation & purification
Nucleic Acid Hybridization
Peptidoglycan / chemistry
Phylogeny*
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
|
| IF |
2.415
|
| 引用数 |
4
|
| リソース情報 |
| 一般微生物 |
JCM 31185
JCM 16176
JCM 16155 |
