| Abstract |
A novel strain, DKC-1T, was isolated from reclaimed grassland soil and was characterized taxonomically by a polyphasic approach. Strain DKC-1T was a Gram-staining-negative, light-yellow-coloured and rod shaped bacterium, motile with polar flagellum. It was able to grow at 20-37 °C, at pH 4.5-9.0 and with 0-3 % (w/v) NaCl concentration. Based on the 16S rRNA gene sequence analysis, strain DKC-1T formed a clade within the members of the genus Dyella and showed highest sequence similarities to Dyella japonica XD53T (98.36 %), Rhodanobacter aciditrophus sjH1T (97.92 %), Rhodanobacter koreensis THG-DD7T (97.74 %), Dyella kyungheensis THG-B117T (97.65 %) and Rhodanobacter terrae GP18-1T (97.40 %). The only respiratory quinone was ubiquinone-8. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidyl-N-methylethanolamine. The predominant fatty acids of strain DKC-1T were iso-C16 : 0, iso-C15 : 0, summed feature 9 (iso-C17 : 1ω9c and/or C16 : 0 10-methyl), iso-C17 : 0, iso-C11 : 0 3-OH and iso-C11 : 0. The genomic DNA G+C content of this novel strain was 63.1 mol%. The DNA-DNA relatedness between strain DKC-1T and its reference strains (D. japonica XD53T, R. aciditrophus sjH1T, R. koreensis THG-DD7T, D. kyungheensis THG-B117T and R. terrae GP18-1T) was 52.3, 44.7, 38.7, 49.0 and 32.7 %, respectively, which falls below the threshold value of 70 % for the strain to be considered as novel. The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished this strain from its closest phylogenetic neighbours. Thus, strain DKC-1T represents a novel species of the genus Dyella, for which the name Dyella agri sp. nov. is proposed. The type strain is DKC-1T (=KEMB 9005-571T=KACC 19176T=JCM 31925T).
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