RRC ID 51177
著者 Xia ZF, Luo XX, Wan CX, Zhang LL.
タイトル Saccharopolyspora aidingensis sp. nov., an actinomycete isolated from a salt lake.
ジャーナル Int J Syst Evol Microbiol
Abstract A novel halophilic actinomycete strain, designated TRM 46074T, was isolated from Aiding salt lake in Turpan Basin, north-west China. Strain TRM 46074T was aerobic, Gram-stain-positive and grew optimally with 12 % (w/v) NaCl. The strain was observed to produce sparse aerial mycelium with long chains of spores that were non-motile and oval or spherical in shape with a rough or smooth surface; substrate mycelium was branched and well developed. The cell wall was determined to contain meso-diaminopimelic acid; the diagnostic whole-cell sugars were galactose, arabinose and ribose. The diagnostic polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol and phosphatidylinositol. The predominant menaquinones were MK-9(H4), MK-10(H8) and MK-10(H4). The G+C content of the DNA was 70.9 mol%. The major fatty acids were anteiso-C17 : 0, iso-C15 : 0, iso-C16 : 0 and iso-C17 : 0. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain TRM 46074T belongs to the genus Saccharopolyspora. Strain TRM 46074T had 16S rRNA gene sequence similarity of 99.6 % with the closest described species, Saccharopolyspora ghardaiensis H53T. DNA-DNA hybridization between strain TRM 46074T and Saccharopolysporaghardaiensis H53T showed 58.6 % relatedness. On the basis of the evidence from a polyphasic study, strain TRM 46074T is considered to represent a novel species in the genus Saccharopolyspora, for which the name Saccharopolyspora aidingensis sp. nov. is proposed. The type strain is TRM 46074T (=CCTCC AA 2012014T=JCM 30185T).
巻・号 67(3)
ページ 687-691
公開日 2017-3-1
DOI 10.1099/ijsem.0.001696
PMID 29144232
MeSH Bacterial Typing Techniques Base Composition China DNA, Bacterial / genetics Diaminopimelic Acid / chemistry Fatty Acids / chemistry Lakes / microbiology* Nucleic Acid Hybridization Phospholipids / chemistry Phylogeny* RNA, Ribosomal, 16S / genetics Saccharopolyspora / classification* Saccharopolyspora / genetics Saccharopolyspora / isolation & purification Salinity* Sequence Analysis, DNA Vitamin K 2 / chemistry
IF 2.415
引用数 1
リソース情報
一般微生物 JCM 30185