Reference - Detail
| RRC ID | 51232 |
|---|---|
| Author | Desaki Y, Kouzai Y, Ninomiya Y, Iwase R, Shimizu Y, Seko K, Molinaro A, Minami E, Shibuya N, Kaku H, Nishizawa Y. |
| Title | OsCERK1 plays a crucial role in the lipopolysaccharide-induced immune response of rice. |
| Journal | New Phytol |
| Abstract |
Plant cell surface receptor-like kinases (RLKs) mediate the signals from microbe-associated molecular patterns (MAMPs) that induce immune responses. Lipopolysaccharide (LPS), the major constituent of the outer membrane of gram-negative bacteria, is a common MAMP perceived by animals and plants; however, the plant receptors/co-receptors are unknown except for LORE, a bulb-type lectin S-domain RLK (B-lectin SD1-RLK) in Arabidopsis. OsCERK1 is a multifunctional RLK in rice that contains lysin motifs (LysMs) and is essential for the perception of chitin, a fungal MAMP, and peptidoglycan, a bacterial MAMP. Here, we analyzed the relevance of OsCERK1 to LPS perception in rice. Using OsCERK1-knockout mutants (oscerk1), we evaluated hydrogen peroxide (H2 O2 ) production and gene expression after LPS treatment. We also examined the LPS response in knockout mutants for the B-lectin SD1-RLK genes in rice and for all LysM-protein genes in Arabidopsis. Compared with wild-type rice cells, LPS responses in oscerk1 cells were mostly diminished. By contrast, rice lines mutated in either of three B-lectin SD1-RLK genes and Arabidopsis lines mutated in the LysM-protein genes responded normally to LPS. From these results, we conclude that OsCERK1 is an LPS receptor/co-receptor and that the LPS perception systems of rice and Arabidopsis are significantly different. |
| Volume | 217(3) |
| Pages | 1042-1049 |
| Published | 2018-2-1 |
| DOI | 10.1111/nph.14941 |
| PMID | 29194635 |
| MeSH | Arabidopsis / drug effects Arabidopsis / genetics Gene Expression Regulation, Plant / drug effects Lipopolysaccharides / pharmacology* Mutation / genetics Oryza / drug effects Oryza / immunology* Plant Cells / drug effects Plant Cells / metabolism Plant Immunity / drug effects* Plant Proteins / genetics Plant Proteins / metabolism* Reactive Oxygen Species / metabolism |
| IF | 8.512 |
| Times Cited | 21 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 22 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Arabidopsis / Cultured plant cells, genes | pst10735 pst11173 pst12599 |