RRC ID 51246
Author Djenoune L, Desban L, Gomez J, Sternberg JR, Prendergast A, Langui D, Quan FB, Marnas H, Auer TO, Rio JP, Del Bene F, Bardet PL, Wyart C.
Title The dual developmental origin of spinal cerebrospinal fluid-contacting neurons gives rise to distinct functional subtypes.
Journal Sci Rep
Abstract Chemical and mechanical cues from the cerebrospinal fluid (CSF) can affect the development and function of the central nervous system (CNS). How such cues are detected and relayed to the CNS remains elusive. Cerebrospinal fluid-contacting neurons (CSF-cNs) situated at the interface between the CSF and the CNS are ideally located to convey such information to local networks. In the spinal cord, these GABAergic neurons expressing the PKD2L1 channel extend an apical extension into the CSF and an ascending axon in the spinal cord. In zebrafish and mouse spinal CSF-cNs originate from two distinct progenitor domains characterized by distinct cascades of transcription factors. Here we ask whether these neurons with different developmental origins differentiate into cells types with different functional properties. We show in zebrafish larva that the expression of specific markers, the morphology of the apical extension and axonal projections, as well as the neuronal targets contacted by CSF-cN axons, distinguish the two CSF-cN subtypes. Altogether our study demonstrates that the developmental origins of spinal CSF-cNs give rise to two distinct functional populations of sensory neurons. This work opens novel avenues to understand how these subtypes may carry distinct functions related to development of the spinal cord, locomotion and posture.
Volume 7(1)
Pages 719
Published 2017-4-7
DOI 10.1038/s41598-017-00350-1
PII 10.1038/s41598-017-00350-1
PMID 28389647
PMC PMC5428266
MeSH Animals Animals, Genetically Modified Axons / physiology Axons / ultrastructure Biomarkers Carrier Proteins / genetics Carrier Proteins / metabolism Cell Differentiation Cerebrospinal Fluid / metabolism* Fluorescent Antibody Technique Ganglia, Spinal Homozygote Mutation Neurons / physiology* Neurons / ultrastructure Sensory Receptor Cells / physiology Sensory Receptor Cells / ultrastructure Signal Transduction* Spinal Cord / cytology* Spinal Cord / physiology* Spinal Nerve Roots TRPP Cation Channels Zebrafish Zebrafish Proteins / genetics Zebrafish Proteins / metabolism
IF 3.998
Times Cited 10
Zebrafish Tg(vglut2a:loxP-DsRed-loxP-GFP)