| RRC ID |
51272
|
| 著者 |
Kim DH, Kim J, Marques JC, Grama A, Hildebrand DGC, Gu W, Li JM, Robson DN.
|
| タイトル |
Pan-neuronal calcium imaging with cellular resolution in freely swimming zebrafish.
|
| ジャーナル |
Nat Methods
|
| Abstract |
Calcium imaging with cellular resolution typically requires an animal to be tethered under a microscope, which substantially restricts the range of behaviors that can be studied. To expand the behavioral repertoire amenable to imaging, we have developed a tracking microscope that enables whole-brain calcium imaging with cellular resolution in freely swimming larval zebrafish. This microscope uses infrared imaging to track a target animal in a behavior arena. On the basis of the predicted trajectory of the animal, we applied optimal control theory to a motorized stage system to cancel brain motion in three dimensions. We combined this motion-cancellation system with differential illumination focal filtering, a variant of HiLo microscopy, which enabled us to image the brain of a freely swimming larval zebrafish for more than an hour. This work expands the repertoire of natural behaviors that can be studied with cellular-resolution calcium imaging to potentially include spatial navigation, social behavior, feeding and reward.
|
| 巻・号 |
14(11)
|
| ページ |
1107-1114
|
| 公開日 |
2017-11-1
|
| DOI |
10.1038/nmeth.4429
|
| PII |
nmeth.4429
|
| PMID |
28892088
|
| MeSH |
Animals
Brain / physiology
Calcium / metabolism*
Microscopy / methods
Neurons / metabolism*
Swimming / physiology*
Zebrafish / physiology*
|
| IF |
30.822
|
| 引用数 |
56
|
| リソース情報 |
| ゼブラフィッシュ |
Tg(HuC:Kaede) |
