論文 - 詳細
| RRC ID | 51272 |
|---|---|
| 著者 | Kim DH, Kim J, Marques JC, Grama A, Hildebrand DGC, Gu W, Li JM, Robson DN. |
| タイトル | Pan-neuronal calcium imaging with cellular resolution in freely swimming zebrafish. |
| ジャーナル | Nat Methods |
| Abstract |
Calcium imaging with cellular resolution typically requires an animal to be tethered under a microscope, which substantially restricts the range of behaviors that can be studied. To expand the behavioral repertoire amenable to imaging, we have developed a tracking microscope that enables whole-brain calcium imaging with cellular resolution in freely swimming larval zebrafish. This microscope uses infrared imaging to track a target animal in a behavior arena. On the basis of the predicted trajectory of the animal, we applied optimal control theory to a motorized stage system to cancel brain motion in three dimensions. We combined this motion-cancellation system with differential illumination focal filtering, a variant of HiLo microscopy, which enabled us to image the brain of a freely swimming larval zebrafish for more than an hour. This work expands the repertoire of natural behaviors that can be studied with cellular-resolution calcium imaging to potentially include spatial navigation, social behavior, feeding and reward. |
| 巻・号 | 14(11) |
| ページ | 1107-1114 |
| 公開日 | 2017-11-1 |
| DOI | 10.1038/nmeth.4429 |
| PII | nmeth.4429 |
| PMID | 28892088 |
| MeSH | Animals Brain / physiology Calcium / metabolism* Microscopy / methods Neurons / metabolism* Swimming / physiology* Zebrafish / physiology* |
| IF | 30.822 |
| 引用数 | 56 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 56 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | Tg(HuC:Kaede) |