RRC ID |
51328
|
著者 |
Rahantaniaina MS, Li S, Chatel-Innocenti G, Tuzet A, Issakidis-Bourguet E, Mhamdi A, Noctor G.
|
タイトル |
Cytosolic and Chloroplastic DHARs Cooperate in Oxidative Stress-Driven Activation of the Salicylic Acid Pathway.
|
ジャーナル |
Plant Physiol
|
Abstract |
The complexity of plant antioxidative systems gives rise to many unresolved questions. One relates to the functional importance of dehydroascorbate reductases (DHARs) in interactions between ascorbate and glutathione. To investigate this issue, we produced a complete set of loss-of-function mutants for the three annotated Arabidopsis (Arabidopsis thaliana) DHARs. The combined loss of DHAR1 and DHAR3 expression decreased extractable activity to very low levels but had little effect on phenotype or ascorbate and glutathione pools in standard conditions. An analysis of the subcellular localization of the DHARs in Arabidopsis lines stably transformed with GFP fusion proteins revealed that DHAR1 and DHAR2 are cytosolic while DHAR3 is chloroplastic, with no evidence for peroxisomal or mitochondrial localizations. When the mutations were introduced into an oxidative stress genetic background (cat2), the dhar1 dhar2 combination decreased glutathione oxidation and inhibited cat2-triggered induction of the salicylic acid pathway. These effects were reversed in cat2 dhar1 dhar2 dhar3 complemented with any of the three DHARs. The data suggest that (1) DHAR can be decreased to negligible levels without marked effects on ascorbate pools, (2) the cytosolic isoforms are particularly important in coupling intracellular hydrogen peroxide metabolism to glutathione oxidation, and (3) DHAR-dependent glutathione oxidation influences redox-driven salicylic acid accumulation.
|
巻・号 |
174(2)
|
ページ |
956-971
|
公開日 |
2017-6-1
|
DOI |
10.1104/pp.17.00317
|
PII |
pp.17.00317
|
PMID |
28381499
|
PMC |
PMC5462045
|
MeSH |
Antioxidants / metabolism
Arabidopsis / enzymology*
Arabidopsis / metabolism
Arabidopsis Proteins / metabolism*
Ascorbic Acid / metabolism
Cell Death
Chloroplasts / enzymology*
Cytosol / enzymology*
DNA, Bacterial / genetics
Genetic Complementation Test
Glutathione / metabolism
Green Fluorescent Proteins / metabolism
Mutagenesis, Insertional / genetics
Mutation / genetics
Oxidative Stress*
Oxidoreductases / metabolism*
Phenotype
Recombinant Fusion Proteins / metabolism
Salicylic Acid / metabolism*
Subcellular Fractions / metabolism
|
IF |
6.902
|
引用数 |
24
|
リソース情報 |
シロイヌナズナ / 植物培養細胞・遺伝子 |
pda10894
pda09951
pda11632 |