RRC ID 51340
Author Papandreou ME, Tavernarakis N.
Title Monitoring Autophagic Responses in Caenorhabditis elegans.
Journal Methods Enzymol
Abstract Autophagy, from the Greek auto (self) and phagy (eating), is a self-degradative process critical for eukaryotic cell homeostasis. Its rapidly responsive, highly dynamic nature renders this process essential for adapting to and offsetting acute/harsh conditions such as starvation, organelle dysfunction, and deoxyribonucleic acid (DNA) damage. Autophagy involves an intricate network of interacting factors with multiple levels of control. Importantly, dysregulation of autophagy has been linked to numerous debilitating pathologies, including cancer and neurodegenerative conditions in humans. Methods to monitor and quantify autophagic activity reliably are essential both for studying the basic mechanisms of autophagy and for dissecting its involvement in disease. The nematode Caenorhabditis elegans is a particularly suitable model organism to effectively visualize and study autophagy, in vivo, in a physiological and pathological context due to its optical transparency, experimental malleability, and precise developmental and anatomical characterization. Here, we outline the main tools and approaches to monitor and measure autophagic responses in C. elegans.
Volume 588
Pages 429-444
Published 2016-12-5
DOI 10.1016/bs.mie.2016.10.039
PII S0076-6879(16)30418-9
PMID 28237114
MeSH Animals Autophagy* Caenorhabditis elegans / cytology* Caenorhabditis elegans / metabolism Caenorhabditis elegans / ultrastructure Caenorhabditis elegans Proteins / metabolism Lysosomes / metabolism Lysosomes / ultrastructure Microscopy, Fluorescence / methods* Mitophagy
IF 1.862
Times Cited 2
C.elegans tm5755