RRC ID 51344
Author Mitani S.
Title Comprehensive functional genomics using Caenorhabditis elegans as a model organism.
Journal Proc Jpn Acad Ser B Phys Biol Sci
Abstract We have been working on functional genomics using C. elegans as a model organism. We first used cell-type specific markers and preexisting mutants to investigate how genotype-phenotype causal relationships are regulated. With the aid of transgenic methods, we analyzed various biological processes in C. elegans. We have developed efficient methods to isolate gene knockout strains. Thousands of strains isolated this way are used by many researchers and have revealed many biological mechanisms. We have also developed methods to examine the functions of genes in a comprehensive manner by integrating transgenes into chromosomes, designing conditional knockouts, and creating balancers for lethal mutations. A combination of these biological resources and techniques will be useful to understand the functions of genes in C. elegans, which has many genes that are orthologous to those of higher organisms including humans.
Volume 93(8)
Pages 561-577
Published 2017
DOI 10.2183/pjab.93.036
PMID 29021508
PMC PMC5743858
MeSH Animals Animals, Genetically Modified Caenorhabditis elegans / genetics* Chromosomes / genetics Gene Expression Profiling / methods Gene Knockout Techniques / methods Genomics / methods* Models, Animal Mutation Transgenes
IF 1.833
Times Cited 2
C.elegans tm698