論文 - 詳細
| RRC ID | 51353 |
|---|---|
| 著者 | Romanelli-Cedrez L, Carrera I, Otero L, Miranda-Vizuete A, Mariotti M, Alkema MJ, Salinas G. |
| タイトル | Selenoprotein T is required for pathogenic bacteria avoidance in Caenorhabditis elegans. |
| ジャーナル | Free Radic Biol Med |
| Abstract |
Selenoprotein T (SELENOT) is an endoplasmatic reticulum (ER)-associated redoxin that contains the amino acid selenocysteine (Sec, U) within a CXXU motif within a thioredoxin-like fold. Its precise function in multicellular organisms is not completely understood although it has been shown in mammals to be involved in Ca2+ homeostasis, antioxidant and neuroendocrine functions. Here, we use the model organism C. elegans to address SELENOT function in a whole organism throughout its life cycle. C. elegans possess two genes encoding SELENOT protein orthologues (SELT-1.1 and SELT-1.2), which lack Sec and contain the CXXC redox motif instead. Our results show that a Sec→Cys replacement and a gene duplication were two major evolutionary events that occurred in the nematode lineage. We find that worm SELT-1.1 localizes to the ER and is expressed in different cell types, including the nervous system. In contrast, SELT-1.2 exclusively localizes in the cytoplasm of the AWB neurons. We find that selt-1.1 and selt-1.2 single mutants as well as the double mutant are viable, but the selt-1.1 mutant is compromised under rotenone-induced oxidative stress. We demonstrate that selt-1.1, but not selt-1.2, is required for avoidance to the bacterial pathogens Serratia marcescens and Pseudomonas aeruginosa. Aversion to the noxious signal 2-nonanone is also significantly impaired in selt-1.1, but not in selt-1.2 mutant animals. Our results suggest that selt-1.1 would be a redox transducer required for nociception and optimal organismal fitness. The results highlight C. elegans as a valuable model organism to study SELENOT-dependent processes. |
| 巻・号 | 108 |
| ページ | 174-182 |
| 公開日 | 2017-7-1 |
| DOI | 10.1016/j.freeradbiomed.2017.03.021 |
| PII | S0891-5849(17)30170-3 |
| PMID | 28347729 |
| MeSH | Animals Caenorhabditis elegans / immunology* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cells, Cultured Cysteine / genetics Endoplasmic Reticulum / metabolism* Gene Duplication Immunity, Innate Ketones / administration & dosage Life Cycle Stages Mutation / genetics Neurons / metabolism* Nociception Oxidative Stress Protein Transport Pseudomonas Infections / immunology* Pseudomonas aeruginosa / immunology* Selenoproteins / genetics Selenoproteins / metabolism* Serratia Infections / immunology* Serratia marcescens / immunology* |
| IF | 6.17 |
| 引用数 | 2 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm3763 tm3771 |