RRC ID 51362
Author Chen CH, He CW, Liao CP, Pan CL.
Title A Wnt-planar polarity pathway instructs neurite branching by restricting F-actin assembly through endosomal signaling.
Journal PLoS Genet
Abstract Spatial arrangement of neurite branching is instructed by both attractive and repulsive cues. Here we show that in C. elegans, the Wnt family of secreted glycoproteins specify neurite branching sites in the PLM mechanosensory neurons. Wnts function through MIG-1/Frizzled and the planar cell polarity protein (PCP) VANG-1/Strabismus/Vangl2 to restrict the formation of F-actin patches, which mark branching sites in nascent neurites. We find that VANG-1 promotes Wnt signaling by facilitating Frizzled endocytosis and genetically acts in a common pathway with arr-1/β-arrestin, whose mutation results in defective PLM branching and F-actin patterns similar to those in the Wnt, mig-1 or vang-1 mutants. On the other hand, the UNC-6/Netrin pathway intersects orthogonally with Wnt-PCP signaling to guide PLM branch growth along the dorsal-ventral axis. Our study provides insights for how attractive and repulsive signals coordinate to sculpt neurite branching patterns, which are critical for circuit connectivity.
Volume 13(4)
Pages e1006720
Published 2017-4-1
DOI 10.1371/journal.pgen.1006720
PMID 28384160
PMC PMC5398721
MeSH Actins / genetics Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development Caenorhabditis elegans Proteins / genetics* Cell Polarity / genetics Endocytosis / genetics Frizzled Receptors / genetics* Nerve Tissue Proteins / genetics* Netrins Neurites / metabolism Neurogenesis / genetics* Neurons / metabolism Phosphoproteins / genetics* Wnt Signaling Pathway / genetics beta-Arrestin 1 / genetics*
IF 5.175
Times Cited 11
C.elegans tm1422