RRC ID 51369
Author Serwas D, Su TY, Roessler M, Wang S, Dammermann A.
Title Centrioles initiate cilia assembly but are dispensable for maturation and maintenance in C. elegans.
Journal J Cell Biol
Abstract Cilia are cellular projections that assemble on centriole-derived basal bodies. While cilia assembly is absolutely dependent on centrioles, it is not known to what extent they contribute to downstream events. The nematode C. elegans provides a unique opportunity to address this question, as centrioles do not persist at the base of mature cilia. Using fluorescence microscopy and electron tomography, we find that centrioles degenerate early during ciliogenesis. The transition zone and axoneme are not completely formed at this time, indicating that cilia maturation does not depend on intact centrioles. The hydrolethalus syndrome protein HYLS-1 is the only centriolar protein known to remain at the base of mature cilia and is required for intraflagellar transport trafficking. Surprisingly, targeted degradation of HYLS-1 after initiation of ciliogenesis does not affect ciliary structures. Taken together, our results indicate that while centrioles are essential to initiate cilia formation, they are dispensable for cilia maturation and maintenance.
Volume 216(6)
Pages 1659-1671
Published 2017-6-5
DOI 10.1083/jcb.201610070
PII jcb.201610070
PMID 28411189
PMC PMC5461022
MeSH Animals Animals, Genetically Modified Axoneme / physiology Basal Bodies / metabolism Basal Bodies / physiology* Basal Bodies / ultrastructure Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Caenorhabditis elegans / physiology* Caenorhabditis elegans / ultrastructure Caenorhabditis elegans Proteins / metabolism Centrioles / metabolism Centrioles / physiology* Centrioles / ultrastructure Cilia / physiology Electron Microscope Tomography Microscopy, Fluorescence Microscopy, Video Neurogenesis* Proteolysis Sensory Receptor Cells / metabolism Sensory Receptor Cells / physiology* Sensory Receptor Cells / ultrastructure Time Factors Time-Lapse Imaging
IF 8.891
Times Cited 17
Resource
C.elegans tm2452 tm3067 tm3433 tm3951 tm4927 tm925