Reference - Detail
|Author||Von Stetina SE, Liang J, Marnellos G, Mango SE.|
|Title||Temporal regulation of epithelium formation mediated by FoxA, MKLP1, MgcRacGAP, and PAR-6.|
|Journal||Mol. Biol. Cell|
Accumulation of DLG-1 protein is delayed by ZEN-4, acting in concert with its binding partner CYK-4/MgcRacGAP. Our structure-function analysis suggests that nuclear and kinesin functions are dispensable, whereas binding to CYK-4 is essential, for ZEN-4 function in polarity. Finally, PAR-6 is necessary to localize polarity proteins such as DLG-1 within adherens junctions and at the apical surface, thereby generating arcade cell polarity. Our results reveal that the timing of a landmark event during embryonic morphogenesis is mediated by the concerted action of four proteins that delay the formation of an epithelial bridge until the appropriate time. In addition, we find that mammalian FoxA associates with many epithelial genes, suggesting that direct regulation of epithelial identity may be a conserved feature of FoxA factors and a contributor to FoxA function in development and cancer.
|MeSH||Adherens Junctions / metabolism Animals Caenorhabditis elegans Caenorhabditis elegans Proteins / metabolism* Cell Polarity / physiology Cytoskeleton / metabolism Digestive System / growth & development Epithelial Cells / metabolism Epithelium / metabolism Gene Expression Regulation, Developmental / genetics Kinesin / metabolism* Microtubule-Associated Proteins / metabolism Morphogenesis / physiology Structure-Activity Relationship Trans-Activators / metabolism*|