論文 - 詳細
| RRC ID | 51433 |
|---|---|
| 著者 | Florica RO, Hipolito V, Bautista S, Anvari H, Rapp C, El-Rass S, Asgharian A, Antonescu CN, Killeen MT. |
| タイトル | The ENU-3 protein family members function in the Wnt pathway parallel to UNC-6/Netrin to promote motor neuron axon outgrowth in C. elegans. |
| ジャーナル | Dev Biol |
| Abstract |
The axons of the DA and DB classes of motor neurons fail to reach the dorsal cord in the absence of the guidance cue UNC-6/Netrin or its receptor UNC-5 in C. elegans. However, the axonal processes usually exit their cell bodies in the ventral cord in the absence of both molecules. Strains lacking functional versions of UNC-6 or UNC-5 have a low level of DA and DB motor neuron axon outgrowth defects. We found that mutations in the genes for all six of the ENU-3 proteins function to enhance the outgrowth defects of the DA and DB axons in strains lacking either UNC-6 or UNC-5. A mutation in the gene for the MIG-14/Wntless protein also enhances defects in a strain lacking either UNC-5 or UNC-6, suggesting that the ENU-3 and Wnt pathways function parallel to the Netrin pathway in directing motor neuron axon outgrowth. Our evidence suggests that the ENU-3 proteins are novel members of the Wnt pathway in nematodes. Five of the six members of the ENU-3 family are predicted to be single-pass trans-membrane proteins. The expression pattern of ENU-3.1 was consistent with plasma membrane localization. One family member, ENU-3.6, lacks the predicted signal peptide and the membrane-spanning domain. In HeLa cells ENU-3.6 had a cytoplasmic localization and caused actin dependent processes to appear. We conclude that the ENU-3 family proteins function in a pathway parallel to the UNC-6/Netrin pathway for motor neuron axon outgrowth, most likely in the Wnt pathway. |
| 巻・号 | 430(1) |
| ページ | 249-261 |
| 公開日 | 2017-10-1 |
| DOI | 10.1016/j.ydbio.2017.06.036 |
| PII | S0012-1606(17)30224-5 |
| PMID | 28694018 |
| MeSH | Animals Axons / metabolism* Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / metabolism* Cell Body / metabolism Cell Nucleus / metabolism Endoplasmic Reticulum / metabolism Green Fluorescent Proteins / metabolism HeLa Cells Humans Membrane Proteins / metabolism* Motor Neurons / metabolism* Nerve Tissue Proteins / metabolism* Netrins Neuronal Outgrowth* Wnt Signaling Pathway* |
| IF | 2.896 |
| 引用数 | 0 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm3797 tm4519 tm5039 tm5189 tm5256 tm6465 |