RRC ID 51448
著者 Campbell JC, Chin-Sang ID, Bendena WG.
タイトル A Caenorhabditis elegans Nutritional-status Based Copper Aversion Assay.
ジャーナル J Vis Exp
Abstract To ensure survival, organisms must be capable of avoiding unfavorable habitats while ensuring a consistent food source. Caenorhabditis elegans alter their locomotory patterns upon detection of diverse environmental stimuli and can modulate their suite of behavioral responses in response to starvation conditions. Nematodes typically exhibit a decreased aversive response when removed from a food source for over 30 min. Observation of behavioral changes in response to a changing nutritional status can provide insight into the mechanisms that regulate the transition from a well-fed to starved state. We have developed an assay that measures a nematode's ability to cross an aversive barrier (i.e. copper) then reach a food source over a prolonged period of time. This protocol builds upon previous work by integrating multiple variables in a manner that allows for continued data collection as the organisms shift towards an increasingly starved condition. Moreover, this assay permits an increased sample size so that larger populations of nematodes can be simultaneously evaluated. Organisms defective for the ability to detect or respond to copper immediately cross the chemical barrier, while wild type nematodes are initially repelled. As wild type worms are increasingly starved, they begin to cross the barrier and reach the food source. We designed this assay to evaluate a mutant that is incapable of responding to diverse environmental cues, including food sensation or detection of aversive chemicals. When evaluated via this protocol, the defective organisms immediately crossed the barrier, but were also incapable of detecting a food source. Hence, these mutants repeatedly cross the chemical barrier despite temporarily reaching a food source. This assay can straightforwardly test populations of worms to evaluate potential pathway defects related to aversion and starvation.
巻・号 (125)
公開日 2017-7-26
DOI 10.3791/55939
PMID 28784963
PMC PMC5612601
MeSH Animal Nutritional Physiological Phenomena* Animals Behavior, Animal / drug effects Biological Assay / methods* Caenorhabditis elegans / drug effects Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics Chemotaxis / drug effects Copper / pharmacology* Cues Mutation Receptors, G-Protein-Coupled / genetics Starvation
IF 1.163
引用数 0
リソース情報
線虫 tm1652