論文 - 詳細
| RRC ID | 51453 |
|---|---|
| 著者 | Rodriguez J, Peglion F, Martin J, Hubatsch L, Reich J, Hirani N, Gubieda AG, Roffey J, Fernandes AR, St Johnston D, Ahringer J, Goehring NW. |
| タイトル | aPKC Cycles between Functionally Distinct PAR Protein Assemblies to Drive Cell Polarity. |
| ジャーナル | Dev Cell |
| Abstract |
The conserved polarity effector proteins PAR-3, PAR-6, CDC-42, and atypical protein kinase C (aPKC) form a core unit of the PAR protein network, which plays a central role in polarizing a broad range of animal cell types. To functionally polarize cells, these proteins must activate aPKC within a spatially defined membrane domain on one side of the cell in response to symmetry-breaking cues. Using the Caenorhabditis elegans zygote as a model, we find that the localization and activation of aPKC involve distinct, specialized aPKC-containing assemblies: a PAR-3-dependent assembly that responds to polarity cues and promotes efficient segregation of aPKC toward the anterior but holds aPKC in an inactive state, and a CDC-42-dependent assembly in which aPKC is active but poorly segregated. Cycling of aPKC between these distinct functional assemblies, which appears to depend on aPKC activity, effectively links cue-sensing and effector roles within the PAR network to ensure robust establishment of polarity. |
| 巻・号 | 42(4) |
| ページ | 400-415.e9 |
| 公開日 | 2017-8-21 |
| DOI | 10.1016/j.devcel.2017.07.007 |
| PII | S1534-5807(17)30549-X |
| PMID | 28781174 |
| PMC | PMC5563072 |
| MeSH | Animals Caenorhabditis elegans / embryology Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism Cell Polarity* Cyclic AMP-Dependent Protein Kinases / genetics Cyclic AMP-Dependent Protein Kinases / metabolism* GTP-Binding Proteins / genetics GTP-Binding Proteins / metabolism HEK293 Cells Humans Protein Binding Protein Serine-Threonine Kinases / genetics Protein Serine-Threonine Kinases / metabolism* Zygote / metabolism |
| IF | 10.092 |
| 引用数 | 51 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 20 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm2616 |