RRC ID 51498
Author Loveless T, Qadota H, Benian GM, Hardin J.
Title Caenorhabditis elegans SORB-1 localizes to integrin adhesion sites and is required for organization of sarcomeres and mitochondria in myocytes.
Journal Mol Biol Cell
Abstract We have identified and characterized sorb-1, the only sorbin and SH3 domain-containing protein family member in Caenorhabditis elegans SORB-1 is strongly localized to integrin adhesion complexes in larvae and adults, including adhesion plaques and dense bodies (Z-disks) of striated muscles and attachment plaques of smooth muscles. SORB-1 is recruited to the actin-binding, membrane-distal regions of dense bodies via its C-terminal SH3 domains in an ATN-1(α-actinin)- and ALP-1(ALP/Enigma)-dependent manner, where it contributes to the organization of sarcomeres. SORB-1 is also found in other tissues known to be under mechanical stress, including stress fibers in migratory distal tip cells and the proximal gonad sheath, where it becomes enriched in response to tissue distention. We provide evidence for a novel role for sorbin family proteins: SORB-1 is required for normal positioning of the mitochondrial network in muscle cells. Finally, we demonstrate that SORB-1 interacts directly with two other dense body components, DEB-1(vinculin) and ZYX-1(zyxin). This work establishes SORB-1 as a bona fide sorbin family protein-one of the late additions to the dense body complex and a conserved regulator of body wall muscle sarcomere organization and organelle positioning.
Volume 28(25)
Pages 3621-3633
Published 2017-12-1
DOI 10.1091/mbc.E16-06-0455
PII mbc.E16-06-0455
PMID 28978740
PMC PMC5706990
MeSH Actinin / metabolism Actins / physiology Animals Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / metabolism Cell Adhesion / physiology Cytoskeleton / metabolism Focal Adhesions / metabolism Integrins / metabolism Microfilament Proteins / metabolism* Mitochondria, Muscle / metabolism Muscle Cells / metabolism* Muscle Proteins / metabolism Phenotype Sarcomeres / metabolism* Vinculin / metabolism Zyxin / metabolism
IF 3.905
Times Cited 0
Resource
C.elegans tm2803