論文 - 詳細
| RRC ID | 51513 |
|---|---|
| 著者 | Borgen MA, Wang D, Grill B. |
| タイトル | RPM-1 regulates axon termination by affecting growth cone collapse and microtubule stability. |
| ジャーナル | Development |
| Abstract |
Axon termination is essential for efficient and accurate nervous system construction. At present, relatively little is known about how growth cone collapse occurs prior to axon termination in vivo Using the mechanosensory neurons of C. elegans, we found collapse prior to axon termination is protracted, with the growth cone transitioning from a dynamic to a static state. Growth cone collapse prior to termination is facilitated by the signaling hub RPM-1. Given the prominence of the cytoskeleton in growth cone collapse, we assessed the relationship between RPM-1 and regulators of actin dynamics and microtubule stability. Our results reveal several important findings about how axon termination is orchestrated: (1) RPM-1 functions in parallel to RHO-1 and CRMP/UNC-33, but is suppressed by the Rac isoform MIG-2; (2) RPM-1 opposes the function of microtubule stabilizers, including tubulin acetyltransferases; and (3) genetic epistasis suggests the microtubule-stabilizing protein Tau/PTL-1 potentially inhibits RPM-1. These findings provide insight into how growth cone collapse is regulated during axon termination in vivo, and suggest that RPM-1 signaling destabilizes microtubules to facilitate growth cone collapse and axon termination. |
| 巻・号 | 144(24) |
| ページ | 4658-4672 |
| 公開日 | 2017-12-15 |
| DOI | 10.1242/dev.154187 |
| PII | dev.154187 |
| PMID | 29084805 |
| PMC | PMC5769622 |
| MeSH | Acetyltransferases / metabolism Actin Cytoskeleton / metabolism Animals Animals, Genetically Modified Axons / physiology* Caenorhabditis elegans / embryology* Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Growth Cones / physiology* Guanine Nucleotide Exchange Factors / genetics* Microtubule-Associated Proteins / metabolism Microtubules / physiology* Nerve Growth Factors / metabolism Nerve Tissue Proteins / metabolism Signal Transduction rac GTP-Binding Proteins / metabolism rho GTP-Binding Proteins / metabolism |
| IF | 5.611 |
| 引用数 | 8 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 10 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm5597 tm597 |