論文 - 詳細
| RRC ID | 51519 |
|---|---|
| 著者 | Lee CS, Lu T, Seydoux G. |
| タイトル | Nanos promotes epigenetic reprograming of the germline by down-regulation of the THAP transcription factor LIN-15B. |
| ジャーナル | Elife |
| Abstract |
Nanos RNA-binding proteins are required for germline development in metazoans, but the underlying mechanisms remain poorly understood. We have profiled the transcriptome of primordial germ cells (PGCs) lacking the nanos homologs nos-1 and nos-2 in C. elegans. nos-1nos-2 PGCs fail to silence hundreds of transcripts normally expressed in oocytes. We find that this misregulation is due to both delayed turnover of maternal transcripts and inappropriate transcriptional activation. The latter appears to be an indirect consequence of delayed turnover of the maternally-inherited transcription factor LIN-15B, a synMuvB class transcription factor known to antagonize PRC2 activity. PRC2 is required for chromatin reprogramming in the germline, and the transcriptome of PGCs lacking PRC2 resembles that of nos-1nos-2 PGCs. Loss of maternal LIN-15B restores fertility to nos-1nos-2 mutants. These findings suggest that Nanos promotes germ cell fate by downregulating maternal RNAs and proteins that would otherwise interfere with PRC2-dependent reprogramming of PGC chromatin. |
| 巻・号 | 6 |
| 公開日 | 2017-11-7 |
| DOI | 10.7554/eLife.30201 |
| PII | 30201 |
| PMID | 29111977 |
| PMC | PMC5734877 |
| MeSH | Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / biosynthesis* Caenorhabditis elegans Proteins / metabolism* Down-Regulation* Epigenesis, Genetic* Gene Expression Profiling Germ Cells / physiology* Transcription Factors / biosynthesis* |
| IF | 7.08 |
| 引用数 | 8 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1489 |